脂肪特异性蛋白27（Fsp27）是一种调控脂肪沉积与代谢的重要因子，其N端结构域能促进脂肪细胞脂滴形成，然而其C端结构域的作用至今仍不明晰。本课题组前期研究结果首次发现其C端有一处错义突变与阿勒泰羊臀脂性高度关联，提示Fsp27基因可能在阿勒泰羊臀脂脂滴形成中扮演重要角色。基于此，本项目以五种脂尾性状类型绵羊群体为研究对象，以发现的Fsp27基因C端错义突变SNP的功能解析为科学问题，探索该SNP与绵羊脂尾性状的关联性及其在脂滴形成中的生物功能。为此，本研究拟分别构建Fsp27基因N、C端截短体以及突变型和野生型Fsp27基因表达载体，转染3T3-L1和293T细胞，并采用荧光定位、活细胞成像技术、脂滴分离、免疫共沉淀等技术检测各种载体对于脂滴融合与大脂滴生成能力的影响。本研究将证实Fsp27 C端及其SNP在绵羊尾脂脂滴形成中的作用，为解析绵羊脂尾性状的遗传机制提供科学依据。

Fat specific protein 27 (Fsp27) is an important factor regulating fat deposition and metabolism with its N-terminal domain promoting the formation of lipid droplets in adipocyte. However, the bio-function role of its C-terminal domain is still unclear. Our previous study revealed that a missense mutation of C-terminal was highly associated with the trait of Altay sheep rump-fat, suggesting Fsp27 gene may be involve in the lipid droplets formation of Altay sheep tail fat. Accordingly, this project will focus on the association analysis between the missense mutation SNP in Fsp27 gene C-terminal and sheep fat tail traits, and elucidate the biological function of Fsp27 gene C-terminal in lipid droplet formation by using five types of tail trait in sheep population. This project will construct the truncated, mutant and wild-type of Fsp27 gene N- and C-terminals expression vector respectively, then transfect 3T3-L1 and 293T cell lines, and finally test the effects of various carriers for fusion and formation of lipid droplet by using some techniques of fluorescence localization, live cell imaging, separation of lipid droplets and immunoprecipitation. The study aims to verify the role of Fsp27 C-terminal and its SNP in the formation of lipid droplets in fat tail, and provide a scientific basis for revealing the genetic mechanism of fat tail trait in sheep.