在无血清条件下，探索人髓性白血病细胞诱导分化生成树突状细胞(DC)方法，观察各种不同诱导条件下DC细胞形态、表型及混合淋巴细胞反应，确定最佳诱导方案。寻找DC体外长时间培养的功能维持和冷冻保存方法，观察DC激活的CTL对自体或异体白血病细胞的细胞毒活性芯緿C对自体骨髓残存白血病细胞净化作用，为DC用于白血病免疫治疗提供理论依据。

Dendritic cells(DCs) are professional antigen presenting cells that play an important role in antitumor immunotherapy. At past time, DCs have been generated from hematopoietic stem cells in bone marrow and monocytes in peripheral blood. This study is differentiation of leukemic DCs from human myelogenous leukemic cells under serum-free condition. Moreover, we also evaluate the effects of Angelica Polysaccharide (APS) in inducing myelogenous leukemic cells into leukemic DCs. DCs were obtained with GM-CSF、IL-4、TNF-α and APS under serum-free condition. The phenotypes of leukemic DCs were detected by flow cytometry. The proliferation of autologous or allogeneic lymphocytes was tested by mixed lymphocytic reaction. Cytotoxicity was examined by LDH-release assay. The results show that the peak of cellular proliferation and viabilities is cultured in 100ug/ml APS under serum-free condition. In APS groups, the expression of CD83、CD80、CD86 and HLA-DR is significant higher than the groups without APS. The proliferation of lymphocytes is more apparent, compared without APS group. The lymphocytes activated by DCs can induce leukemia-specific cytotoxic response. These results indicate that leukemic DCs can be generated from leukemic cells under serum-free condition. APS may improve generation and maturation of DCs from myeloid leukemic cells. This observation gives a good clinical prospect for the immunotherapy of leukemia.