胶原蛋白支架载bFGF基因转染的月经血源子宫内膜干细胞移植治疗大鼠宫腔粘连模型的研究

宫腔粘连严重危害女性生殖功能，目前临床治疗局限性是缺乏活性内膜，粘连分解后复发率高。如何促进子宫内膜再生并恢复生殖功能，是临床上亟待解决的问题。月经血源内膜干细胞（Mb-MSCs）再生能力强，来源丰富且无创，本课题研究Mb-MSCs在体外培养条件下向子宫内膜上皮分化的能力，并建立大鼠的宫腔粘连模型, 借助慢病毒转染技术将成纤维细胞生长因子（bFGF）基因过表达到Mb-MSCs中，通过胶原蛋白支架作为载体将Mb-MSCs移植到IUA模型的大鼠宫腔内，使内膜干细胞在宫腔内持续分泌高剂量外源性bFGF，以研究Mb-MSCs对宫腔粘连的修复作用、潜在的分子机制和对生育能力的改善。本课题将为宫腔粘连采用干细胞联合生物支架的临床治疗提供动物模型基础。

Intrauterine adhesions can seriously damage women’s reproductive function. The limitation of current clinical treatment is the lack of active endometrium, causing the high recurrence rate of adhesion after adhesionlysis. Promoting endometrial regeneration and restoring reproductive function is an urgent problem in clinic. The menstrual blood derived endometrial stem cells (Mb-MSCs) have strong regeneration ability, abundant source and noninvasive obtain. Our research aims to study Mb-MSCs’s differentiation ability to endometrial epithelial cell in vitro. Then we will establish a rat model of intrauterine adhesions. By lentivirus transfection technique, fibroblast growth factor (bFGF) gene will be over expressed into Mb-MSCs, which carried by collagen scaffold, will be ported to uterine cavity of rat IUA model. By high secretion of exogenous bFGF in the uterine cavity ,Mb-MSCs may promote intrauterine endometrial repair and improve fertility. Our research will provide animal model for the clinical use of Mb-MSCs and biological scaffold for the treatment of intrauterine adhesions.