葡萄膜黑色素瘤（UM）是成人最常见的内眼肿瘤，转移率高死亡率高，严重威胁生命健康，揭示其发病机制已成为亟待解决的重要问题。UM病因研究虽已广泛开展，但以长非编码RNA（lncRNA）为代表的表观遗传研究却尚未深入阐明。CASC15是与肿瘤发生相关的lncRNA，然而其功能尚存在争议，作用机制仍不明确。项目组前期证实CASC15在UM中存在一个新转录产物，将其命名为CASC15-UM1（UM1），UM1 在UM中表达显著下降，UM1过表达能降低UM的转移能力，促进靶基因XIST表达。据此本项目提出了UM1 介导XIST基因调控UM发生的机制假设。本项目将以新lncRNA转录本为突破口，采用特色的表观遗传技术手段，探究UM1对XIST的表观遗传修饰，揭示参与修饰的关键蛋白，阐述UM中lncRNA的调控特点，期望为UM的机制研究拓展思路，为lncRNA参与的其他类型疾病的研究提供有益的借鉴。

As the most common intraocular malignant tumor, uveal melanoma (UM) has the high metastasis and mortality rate, which makes it an urgent problem to reveal its pathogenesis. Studies on traditional genetics are widely made, but mechanisms on epigenetics, especially on long non-coding RNA (lncRNA) remain not clear. CASC15 lncRNA is reported to be closely related to tumorigenesis. However its function is controversial between different types of tumors, and the underlying mechanism is not well expounded. In preliminary work, we identified a novel transcription CASC15-UM1 (UM1) from UM. It was confirmed that the level of UM1 lncRNA was significantly decreased in UM cells and tissues. Over-expressed UM1 lncRNA could reduce the capability of migration of UM cells and activate expression of target gene XIST.Based on these findings, we propose the hypothesis that UM1 lncRNA determines UM tumorigenesis by modification of XIST gene expression.As the breakthrough point of the novel lncRNA transcription and epigenetics technology, this project aims to explore UM1 lncRNA epigenetics modification of XIST promoter, reveal the key protein factor during modification, expound the regulation feature of lncRNA in UM, as well as provide thinking and reference for researches on other lncRNA revolved diseases.