肌内脂肪沉积是影响动物肉质的重要因素。研究肌内脂肪细胞分化是阐明肌内脂肪沉积的重要途径，脂肪细胞分化是一系列转录调控因子协同作用的结果。申请人前期试验显示KLF4、KLF10和KLF15是调控山羊肌内脂肪沉积的关键基因，因此本项目以体外培养的山羊肌内前体脂肪细胞为研究对象，利用转录组测序、RNA干扰、超表达、免疫共沉淀、qPCR和Western Blot等技术阐明转录因子KLF4、KLF10和KLF15对山羊肌内脂肪细胞分化的调控作用，构建KLF家族细胞分化协同调控网络，并分析KLFs通过调控靶标基因的表达影响山羊肌内脂肪细胞分化的生物学效应。以期揭示KLFs调控山羊肌内前体脂肪细胞分化的分子机制。其结果为进一步阐明山羊肌内脂肪沉积机理提供理论依据，为动物肉品质改善提供新思路。

Intramuscular fat (IMF) deposition is a vital contributor to animal meat quality. Intramuscular adipocyte differentiation, which is synergistically regulated by several transcription factors, is a critical step in IMF deposition. Our previous result revealed that KLF4, KLF10 and KLF15 were the key genes related to IMF deposition of goat. In the present study, the role of KLF4, KLF10 and KLF15 in regulating intramuscular adipocyte differentiation of goat will be explored by RNA-Seq, RNA silencing, gene overexpression, co-immunoprecipitation, qPCR and western blot. Furthermore, a synergistical regulatory network of KLFs and their partners controling goat intramuscular adipocyte differentiation will be established. These data will provide a therotically basis for clarifying the mechanism of goat IMF deposition, and improving goat meat quality in the future.