金纳米棒（AuNRs）的表面修饰赋予生物医学应用中的优异性能，但其安全性也引起密切关注。细胞学效应的研究能对不同表面性质AuNRs的安全性预测和评价，一般需从AuNRs与蛋白质、生物膜等生物界面作用等基本问题着手。但是，传统的研究手段难以获得AuNRs-生物界面的精细结构，限制了细胞学效应的研究。因此，本项目拟充分利用同步辐射分析方法的高分辨结构解析能力，结合常规纳米生物学方法，研究表面理化性质对AuNRs-生物界面作用及细胞学效应的调控机制。目标是用同步辐射技术获取界面作用的关键结构信息；通过纳米表征、分子细胞学技术、显微成像、元素定量分析等技术，研究界面结构与性质及其对细胞学效应的影响；并通过理论模拟刻画界面作用的分子过程，揭示表面性质调控细胞学效应的可能机制，为AuNRs的安全与合理应用提供科学依据和理论指导。本项目将建立研究细胞学效应的新方法，为纳米-生物界面研究提供参考与借鉴。

During applying in biomedicine, surface modification provides more excellent properties of gold nanorods(AuNRs). However, the issue about safety of AuNRs has been raised and gets close attentions. With different surface properties, the safety of AuNRs can be predicted and evaluated according to the study in cellular effects. Importantly, this study should base on the interaction between AuNRs and bio-interfaces including proteins and biomembranes. Nevertheless, the fine structure between AuNRs and bio-interfaces is hard to be obtained according to traditional techniques, which restrain further understanding of cellular effect mechanism. As we know, synchrotron radiation(SR)-based analytical techniques can provide fine structure information of high resolution. Therefore, in this proposal, we plan to apply SR-based analytical techniques and usual approaches in nanobiology research in studying how surface physicochemistry mediate interaction between AuNRs and biointerfaces and following cellular responses. As a result, we hope to obtain the central information about nano-biointerfaces using SR-based analytical techniques. Based on the above information, we will also take advantage of routine techniques for nanomaterial characterization, molecular cell biology, and element quantitation, and macroscopic techniques to study the structures and properties of nano-biointerface and following cellular effects. Furthermore, we will use molecular dynamics models to describe the molecular events happened at interfaces. Finally, we will try to explain how surface physicochemical properties mediate interaction between proteins, membranes and AuNRs and the possible mechanism about surface effects of AuNRs on cells. In conclusion, the research will probably provide basic and important evidences which are instructive to understand and evaluate safety of nanomaterials. It will also build up a novel approach to investigate cellular effects and contribute to related studies about the interaction between nanomaterials and biointerfaces.