蝙蝠腺病毒感染后蝙蝠细胞的免疫反应及病毒转录组分析

蝙蝠是多种新发病毒的自然宿主，然而被病毒感染的蝙蝠却罕见发病，其中的机制未知。申请人实验室近期构建了蝙蝠细胞系，并分离出一株蝙蝠腺病毒，为研究蝙蝠与宿主的相互作用提供了条件。本项目在前期蝙蝠干扰素工作基础上，结合高通量测序和定量PCR技术，研究蝙蝠腺病毒感染后蝙蝠细胞内天然免疫反应及病毒转录组变化。目的是从基因转录水平上揭示蝙蝠腺病毒与其自然宿主天然免疫系统的相互作用机制，研究结果将对未来设计对抗这些病毒的药物提供新的思路。

Bats have been known as the natural reservoir host of many emerging infectious diseases.However, the virus infected bats seldom showed clinial diseases, in which the mechanism behind is still unknown. Recently, one bat cell lines has been sucessefully cultured in our laboratory and used to isolate one novel bat adenovirus. This make it possible for further studying the interaction between the virus and the host. Based on our previous data on bat interferon, this project will focus on cellular innate immune response and viral transcriptome following the infection of bat cells with bat adenovirus,through the high throughput sequencing and quantitative PCR techniques. The aim of this project is to uncover the mechanism of bat innate immunity interacting with bat adenovirus at the transcriptional level. These works may eventually guide the drug designing against bat borne viral dieases.