表面增强拉曼散射（SERS）生物传感技术在研究生物分子的相互作用方面具有独特的优势，高选择性和高灵敏度是该技术迅速发展的保证。采用特异性识别功能的生物分子作为敏感元件为选择性测定提供了基础。然而，常规SERS检测并没有将目标识别事件转换成的响应信号进一步放大，所以无法获得更高的检测灵敏度。本项目拟设计基于酪胺信号放大（TSA）技术的SERS传感器，用于对蛋白质的高灵敏度检测。其原理是通过信号分子沉积过程，将大量SERS探针分子富集到传感器上，导致光谱信号显著增强，使系统灵敏度得到前所未有的提升。SERS方法结合TSA技术可以减少生物分子的消耗量，降低非特异性背景信号的干扰，检测常规方法所检测不到的低浓度样品。该方法能够兼容多种生物识别体系，例如酶与底物结合、抗原抗体反应、核酸原位杂交等，实现对不同生物成分的痕量检测，进而推动SERS分析技术向生物医学研究和临床早期诊断再迈进一步。

Surface-enhanced Raman scattering (SERS) bio-sensing technology has a unique advantage in studying the interactions of biomolecules. High selectivity and high sensitivity are the guarantee of the rapid development of SERS technology. Using the molecules with specific identification function as a sensitive element is the basis for selective determination. However, in conventional SERS detection, there is no further amplification of the response signal from the conversion of the target identification event, therefore, it is impossible to obtain higher detection sensitivity. This project is intended to design a SERS sensor based on tyramine signal amplification (TSA) technology for the highly sensitive detection of proteins. Through the process of signal molecular deposition, a large number of SERS probe molecules can be enriched to the sensor, resulting in a significant increase in spectral signal, and the system sensitivity will have an unprecedented improvement. SERS method combined with the TSA technology can reduce the consumption of the biomolecules, reduce the interference of non-specific background signal, and detect a low concentration sample that can't be detected by conventional methods. This method can be compatible with many identified and functional systems, such as enzyme and substrate binding, antigen-antibody reaction, nucleic acid in situ hybridization etc. achieving the trace detection of different biological components, promoting SERS analysis technology to a further step in biomedical research and clinical early diagnosis.