橘色藻（Trentepohlia aurea）属于高山气生藻类，能在低温冷冻、干燥雾霭等多变和极端条件下生长，根据我们的初步结果推测其具有特殊的逆境胁迫适应机制，但是其适应极端逆境胁迫的特殊生理生态特征及分子调控机制尚不清楚。针对这一问题，本申请对比研究低温（包括零上低温和冷冻）、干燥胁迫下橘色藻光合、呼吸等代谢的运行规律，揭示其能够在极端逆境胁迫下维持正常代谢的生理调节机制，并在此基础上，着重阐述其适应低温、干燥胁迫的独特生理生态特征；同时利用转录组和数字基因表达谱技术，鉴定橘色藻中与适应低温、干燥胁迫相关的保护基因和调控基因，揭示该藻可能存在的适应逆境胁迫的独特分子调控机制。目的是全面深入地了解该橘色藻所特有的基础生物学特征与规律，丰富气生藻与植物生理学的研究内容，也为人工开发橘色藻资源积累基础资料，并以期为遗传改良某些植物以提高其抗逆性提供新的思路。

It has been showed that the aeroterrestrial microalgae are tolerant of stresses. But it remains unclear the physiological, ecological and molecular mechanisms of the adaptation to stresses of aeroterrestrial microalgae. In this study, the changes of photosynthetic and respiratory metabolisms in Trentepohlia aurea under chilling and desiccation stresses will be studied. The physiological and ecological mechanisms of the adaptation to chilling or desiccation of Trentepohlia aurea will be systemically researched. The special physiological mechanisms of the adaptation to stresses in Trentepohlia aurea will be analyzed. The key genes related to chilling or desiccation adaptation in Trentepohlia aurea will be found by comparison of control and stresses transcriptome database. And the molecular mechanisms of the adaptation to chilling or desiccation of Trentepohlia aurea will be confirmed. It will provide a more comprehensive rationalization of the mechanism of the adaptation to chilling or desiccation in aeroterrestrial microalgae, and provide theoretical and technical support for the application of Trentepohlia aurea. And it will benefit to the enhancement of the stresses tolerance in plants through the optimization of medium or genetic engineering methods.