研究基于光纤光学的生物对象间接微操控系统，避免直接光学微操控过程中已发现的一系列细胞或亚细胞组分的光致损伤，开创性地应用光纤及微光学元件产生的光纤低模式LP21模光束特点，即高度相干的四瓣形光束可由连接光纤旋转而转动四瓣形能量分布，由光纤直接导入微流道中，对高聚物间接粒子实现空间微操控，包括3D平移，轴向旋转和随光纤倾斜的完整空间六个自由度，开辟微纳光学在间接微操控中的全新应用，使间接微操控从体积大，且振动敏感的自由空间光路系统上升至光波导型系统，领先进入生物纳米光学应用的新领域。采用3D-FTDT等理论工具研究与LP21四瓣形光束相匹配的间接粒子结构设计，优化光阱的各运动刚性，并进一步同时运用多个光纤完成多生物对象之间的微操控过程。高分辨光纤微光学微操控在生物医学和生命科学各个领域具有广阔的应用前景，在推进基础研究和各类生物医学应用中具有重大意义。

This research focuses on an indirect micromanipulation system for biological objects based on fiber-optics, completely avoiding photo-damages caused by laser exposure in direct optical micromanipulations. The system pioneers the application of a low-mode in optical fiber, i.e. LP21 mode, which features a beam consisted of four-lobed energy distributions with a high coherence. It is of great advantage to indirect micromanipulation that the optical trap formed by the four-lobed beam is capable of executing 3D translation, axial rotation and yaw/pitch tilt of indirect particle with complete six degrees of freedom simply by using fiber movement or rotation of connecting fiber, which opens up new applications of micro/nano optics in bioengineering. It is promising that the indirect micromanipulation system will transform from an existing bulky and vibration-vulnerable free-space optical system into a waveguide based compact system. Study will also be conducted on the design of indirect particle structures using 3D FTDT theoretical model to match LP21 beam characteristics, in an effort to optimize stiffness of optical trap and further use of multiple fibers for micromanipulation processes among biological objects. The high resolution fiber-optics based micromanipulation has broad application prospects in both biomedical and life sciences, and is of great significance in promoting basic research and biomedical applications.