除了参与中心体成熟和两极纺锤体形成，细胞周期调控激酶Aurora-A在多种肿瘤中过表达，促进肿瘤恶性进展。研究发现促癌基因广泛参与肿瘤代谢重编程的调控，但Aurora-A和肿瘤代谢的关系还知之甚少。我们前期研究发现Aurora-A可以通过磷酸化乳酸脱氢酶B，增强糖酵解和生物合成，促进肿瘤细胞S-G2期进程（Nat.Comm.2019）。近期，我们还发现一个代谢支路，磷酸戊糖途径可以调节分裂期Aurora-A的活性，且其代谢产物NADPH和Aurora-A能发生直接的相互作用，暗示磷酸戊糖途径可能通过NADPH调节分裂期Aurora-A的活性。因此，在本课题中我们计划深入研究NADPH和分裂期Aurora-A活性的关系，进而揭示磷酸戊糖途径及其代谢物NADPH在细胞分裂和肿瘤进展中新的调控机制。本研究有助于深入理解肿瘤代谢状态对于分裂进程及染色体精准分离的重要意义，可为肿瘤的诊疗提供新思路。

In addition to participating in centrosome maturation and bipolar spindle formation, the cell cycle-regulated kinase Aurora-A is overexpressed in a variety of tumors and can promote tumorigenesis and tumor progression. It is reported that oncogenes are widely involved in the regulation of tumor metabolism reprogramming. However, little is known about the relationship between Aurora-A and tumor metabolism. Our previous studies found that Aurora-A can promote glycolysis, bio-synthesis and S-G2 progression by phosphorylation of LDHB(Nat.Comm.2019). Recently, we also found that a branch of sugar metabolism, the pentose phosphate pathway can specifically regulate the kinase activity of Aurora-A in mitosis, and its metabolite NADPH can bind to Aurora-A directly, indicating that the pentose phosphate pathway may regulate the activity and function of Aurora-A through NADPH. Therefore, in this project, we plan to study the relationship between NADPH and the activity of Aurora-A kinase in cell division, and reveal the new functions of the pentose phosphate pathway and its metabolite NADPH in the regulation of cell division and tumor progression. This study is helpful for understanding the significance of cell metabolism for timely and accurate segregation of chromosomes, and can provide new ideas for the diagnosis and treatment of tumors.