微生物-电动联合修复技术可通过微生物降解与电化学氧化之间的协同效应促进土壤中石油烃类污染物的去除，但修复中后期污染物去除的高效性难以维持。究其原因，可能随着修复的进行，微生物对土壤异种来源有机碳代谢方式改变所致，而这种改变可能由土壤微环境和微生物代谢状态变化引起。本研究通过实验室模拟，以二维对称电场为修复平台，以微生物修复、电动修复和微生物-电动联合修复为研究基础，采用13C稳定同位素示踪技术和高通量测序等分子生物学手段监测不同处理方式下土壤异种来源有机碳代谢利用模式和微生物群落结构、丰度等动态特征，借以探索、分析土壤有机碳代谢机制和微生物代谢状态，结合土壤微环境时空变化特征，揭示微生物-电动修复中后期制约污染物持续高效降解的关键因素，并探索其机制，在此基础上建立微生物-电动联合修复技术的过程调控方法。研究结果将为进一步提高微生物-电动协同修复效率提供理论依据。

The removal of petroleum hydrocarbons could be enhanced by a synergistic effect between biological degradation and electrochemical oxidation during the bio-electrokinetics (BIO-EK) treatment, but the efficiency of pollutant removal is difficult to maintain in the subsequent phase. It probably due to the variation of metabolic patterns of heterogeneous source of organic carbons by microorganism, which may be caused by the changes of soil micro-environmental factors and microbial metabolic status. Through laboratory simulation tests with a 2-D electric field as remediation platform, and on the basis of the technologies of bioremediation (BIO), electrokinetics (EK) and BIO-EK, the metabolic patterns of different carbon sources and the dynamic characteristics of microbial community structure and abundance will be detected by 13C stable isotope tracer technique and molecular biological methods such as high-throughput sequencing, respectively, thus to explore and analyze the metabolism mechanism of organic carbons and microbial metabolic status, combining with the spatio-temporal variation characteristics of soil micro-environment, trying to get access to the key factors that restrict the efficient degradation of petroleum hydrocarbons in the subsequent phase of BIO-EK, the effect mechanism will thus be explored, and a process regulation strategy of the technology of BIO-EK will eventually be determined. The research results will provide a theoretical foundation for improving the efficiency of the technology of BIO-EK.