放射性视神经病变（RION）是肿瘤放射治疗后的常见并发症, 也是放射线暴露突发事件中，可导致失明的严重放射性损伤。如何能在放射暴露后对RION进行实时监测并早期诊断，是降低RION危害性的关键，也是目前制约RION有效治疗的难题。近年来，筛选非编码微小RNA（miRNAs）作为特定疾病发生发展的分子标志物已成为研究热点。本课题前期应用miRNAs微阵列芯片，在体外成功筛选出了视神经放射损伤后差异表达的12个miRNAs，在此基础上将进一步建立动物模型，检测RION不同发展阶段外周血、眼房水和玻璃体中特异性miRNAs的表达规律，尝试找到可以作为RION分子标志物的miRNAs，并分析和鉴定其靶基因，探讨miRNAs及其靶基因在RION产生中的生物学作用。研究结果将不仅解决放疗后RION实时监测和早期诊断问题，而且在职业放射暴露和放射突发事件时，可作为一项快速有效的视神经损伤检测生物指标。

Radiation-induced optic neuropathy（RION）is a common ocular complication induced by the treatment beam for tumors.RION is also an severe radiation damage which can cause blindness after human exposure to radiation.The key point to reduce the severe damage of RION is how to realtime detection and early diagnosis for RION after radiation exposure. However, there are still without effective methods for RION early detection and diagnosis, which causes the result of RION associated visual loss. For the past few years,screening special small non-coding RNAs (miRNAs) as biomarkers for diseases has attracted many scientists' attention.Using miRNAs micoarray analysis in our previous study,we have screened the changes of 12 miRNAs expression in cultured ganglion cells following radation exposure in vitro. Based on the previous results,we will establish the stable RION research animal model. Expression of special miRNAs related to RION in circulating blood,aqueous humor and vitreous will be detected at different periods of RION. The aim of the project is to find some special miRNAs which can act as RION molecular biomarkers,and also analyze the biological functions of these miRNAs and their tagart genes on RION occurrence and development.Through this study,we will provide the experimental basis of miRNAs biomarkers for RION realtime detection and early diagnosis,and miRNAs biomarkers analysis also can be used as a rapid and effective method to detect optic nerve injury after radiation occupational exposure and radiation events.