基于HIF/VEGF/Notch信号通路对非血管化输尿管段自体移植后缺氧诱导血管新生的作用机制及模式的实验研究

非血管化自体组织用于输尿管重建的工作近来受到关注。我们在前期工作中成功建立了非血管化输尿管段自体移植动物模型，并发现输尿管段血管新生可能的两种模式：横向模式与纵向模式。然而非血管化输尿管段在缺氧诱导下发生血管新生从而存活的机制尚待研究。本项目拟在前期输尿管重建动物模型的基础上，主要通过细胞学与组织学实验探讨HIF/VEGF/Notch信号通路分子在缺氧刺激下的表达情况，从而阐明该信号通路在缺氧诱导非血管化输尿管段血管新生过程中的作用机制。同时通过对比输尿管段不同节段组织中HIF/VEGF/Notch信号通路分子的表达水平，探讨其血管新生的可能模式。本项目可为输尿管重建手术后组织修复与功能重建的改善提供理论依据，对开拓输尿管损伤治疗的新思路具有重要意义。

Using non-vascularized autologous graft in ureteral reconstruction has recently received great attention. In the previous study, we successfully established the animal model of autotransplantation of non-vascularized ureteral segment, and found two potential modes of angiogenesis in ureteral tissue, including the transverse mode and the longitudinal mode. However, the mechanism of hypoxia-induced angiogenesis and the consequent survival of non-vascularized ureteral segment call for further investigation. On the basis of the previous animal model of ureteral reconstruction, this project focuses on the expression of HIF/VEGF/Notch signaling pathway under hypoxia through cytological and histological experiments, so as to clarify its mechanism in ureteral angiogenesis. Meanwhile, by comparing the expression level of HIF/VEGF/Notch pathway in tissue of different ureteral segments, the potential modes of angiogenesis also will be discussed. This project can provide theoretical reference for improvement of postoperative tissuel repair and functional reconstruction. It has profound value for exploring new methods of treatment for ureteral injuries.