开环聚合构筑酶响应性聚氨基酸基荧光探针

从响应性高分子材料设计的角度，完善和拓展荧光探针的功能，不仅能够更好的理解高分子本身的结构与功能的关系，还能推动刺激响应性高分子在医学检测平台技术的发展。整合小分子荧光探针原位诊断的优势，以及酶刺激响应聚氨基酸材料的结构响应特性，本项目拟采用活性N-羧酸酐（NCA）可控开环聚合并结合后修饰等手段，制备酶刺激响应性聚肽基荧光探针。我们将通过分子设计和组装，调控聚肽高分子的酶刺激响应性与荧光探针功能的集成与协同，不断优化结构，探索酶响应性聚肽基荧光探针在肿瘤诊断的应用。

Improving and expanding the in vivo function of small-molecule fluorescent probes by integrating with the stimuli-responsive polymer, which could help us understand the relationship between structure and function of polymer, and promote the development of the responsive polymer in the field of medical detection and diagnosis. In this proposal, we aim to integrate the enzyme-responsive polypeptides with the enzyme-responsive small-molecule fluorescent probe. The enzyme-responsive polypeptides will be prepared via controlled ring opening polymerization (ROP) of amino acid N-carboxyanhydride (NCA) monomer and post-polymerization modification of side chains. The effects of enzyme-responsive polypeptides chain length, secondary structure, hydrophobicity-hydrophilicity ratio, and polymeric assembly will be investigated systematically on the enzyme-responsibility of small-molecule fluorescent probes. The coordination mechanism of polypeptides-based fluorescent probes response to enzyme will be addressed to increase the sensitivity and selectivity. The obtained information will guide us the new polypeptides design and synthesis to optimize the enzyme-response properties of polypeptide-based fluorescent probes. Finally, the enzyme-responsive polypeptides- based fluorescent probe will be further investigated as diagnosis & therapy systems for tumor.