由于其独特的光化学和光物理性质，绿荧光蛋白色素p-HBDI及其衍生物被广泛用于荧光探针和传感器探测金属离子、DNA以及pH等，研究这些衍生物的激发态性质和动力学并与p-HBDI进行对比，将有助于人们进一步了解p-HBDI的内转化和光致异构化过程的本质，对人们进一步修饰和改良p-HBDI的结构，调控其光化学和光物理性质，起到一定的指导意义。本项目将利用超快时间分辨光谱实验、高精度量子化学计算与非绝热动力学模拟相结合的方式，对绿荧光蛋白色素p-HBDI的各种衍生物的激发态动力学进行研究，考察取代基及其位置、溶剂极性以及氢键对p-HBDI衍生物的光化学和光物理性质的影响，尤其是溶质溶剂间分子间氢键对光致异构化效率和荧光效率的影响，以及溶质溶剂间分子间氢键与分子内氢键的竞争机制。

Owing to the unique photochemical and photophysical properties, the green fluorescent protein chromophore and its derivatives has been used widely as fluorescent probes and sensors to detect metal ion, DNA, and PH, etc. Studying the excited state properties and dynamics of the derivatives of p-HBDI and comparing with those of the p-HBDI will help the researcher further understand the nature of interal conversion and photoisomerization of the p-HBDI, and guide the researcher to further improve the properties of the derivatives of p-HBDI. In this project, we will use the ultrafast time-resolved spectroscopy, high precision quantum chemistry calculations, and nonadiabatic dynamics to study the excited state dynamics of the derivatives of p-HBDI. We will investigate the effects of substitutes and their positions, solvent polarity, and hydrogen boding on the photochemical and photophysical properties of the derivatives of p-HBDI, especially, the effects of solute-solvent hydrogen bonding on the efficiencies of photoisomerization and fluorescence quantum yield, and the competition between the intermolecular and intramolecular hydrogen bonding.