两栖类皮肤及其分泌物中含有丰富的抗菌肽类物质，具有极高的临床应用价值，是国际上的研究热点。本项目拟以四川湍蛙（Amolops mantzorum）为研究对象，综合运用生态学、生物化学、分子生物学、微生物学、定量蛋白质组学和多肽组学等交叉学科的方法和技术，重点探查其不同地理种群间皮肤分泌物中含有抗菌肽的结构特异性，结合其在抵御不同微生物侵袭时所分泌的各家族抗菌肽在转录水平和翻译水平上的动态变化，解释其分子机制。对于揭示造成两栖类抗菌肽多样性的诱因，阐明两栖类对环境适应性的生理机制，以及两栖类与微生物的协同进化等均具有重要意义，并对两栖类疾病的早期诊断以及应用这些成果建立抗菌肽在两栖类中的定向诱导等方面提供依据。

The amphibian skin and its secretion contain rich antimicrobial peptides with very high value for clinical application, and hence a hot spot of research in the world. This project plan to study Amolops mantzorum using the methods and techniques in ecology, biochemistry, molecular biology, microbiology, quantitative proteomics, and peptidomics. The research focus is the structural specificity of antimicrobial peptides in the skin secretion of different geological species of A. mantzorum, and the dynamic changes in the transcriptional and translational level of different families of antimicrobial peptides secreted against different microbial attacks. This is important to uncover the reason for the diversity of amphibian antimicrobial peptides, to elucidate the physiological mechanisms for amphibians to adapt to environment, and to understand the cooperative evolution of amphibians and microbes. It also provides the clues to the diagnosis of amphibian diseases at early stage, and help to use this result to construct directional inducement of antimicrobial peptides in amphibians.