骨缺损是骨科临床常见病和难治病，尤以长骨大段骨缺损修复最为困难。钛合金凭借优良生物相容性和抗腐蚀性成为理想的骨替代修复材料。由于传统钛合金与骨弹性模量不匹配，课题组研制了新型低弹多孔钛合金，该材料能在骨小梁水平实现弹性模量匹配，具有较好的生物相容性。但它在微观水平仍难与骨组织实现充分整合，而对骨质疏松患者骨整合则更为困难。骨处于复杂的应力和电磁微环境中，诸多研究证实脉冲电磁场和振动波具有促骨生成效果，且二者作用机制不同。因此本研究提出了脉冲电磁场、振动波协同低弹多孔钛合金修复骨缺损的新理念，通过施加脉冲电磁场和振动波为多孔钛合金的修复区域构建应力-电磁微环境，通过体外和体内实验系统评估电磁场、振动协同多孔钛合金对骨骼细胞生长和骨缺损修复的作用效果，筛选最佳电磁场、振动波参数，并以细胞钙信号为切入点探索作用的相关机制。本研究旨在为临床骨缺损修复提供新思路，降低骨缺损治疗成本，改善病患生活质量

Repair of bone defects is one of the most common and toughest issues in orthopedic surgery, and especially thorny for the segmental bone defects repair in long bone. Titanium alloy has become an ideal bone substitutes to repair bone defects, based on its excellent biocompatibility and corrosion-resistant capacity. Due to the mismatching of elastic modulus between the traditional titanium alloy and bone, our group has designed a novel porous titanium alloy with low elastic modulus, which exhibited significant similarity in elastic modulus with trabecular bone as well as favorable biocompatibility. However, the poor osseointegration of this porous titanium alloy for patients with bone defects was a non-negligible issue, especially for the osteoporotic patients. The skeletal tissues are surrounded by complex mechanical and electromagnetic microenvironment. Substantial studies have shown that both pulsed electromagnetic fields (PEMF) and whole-body vibration (WBV) were able to promote bone healing, whereas PEMF and WBV displayed distinct osteogenetic mechanisms. Thus, in this proposal, we hypothesize that synergetic application of our new porous titanium alloy with PEMF and WBV may generate more significant efficiency in bone defects repair by establishing the 'mechanical-electromagnetic' microenvironment surrounding the porous titanium alloy. We will systematically evaluate the effects of synergetic application of porous titanium alloy with PEMF and WBV on the functions of bone cells in vitro and bone defect repair in vivo, as well as achieving the optimum stimulation parameters of PEMF and WBV. Moreover, we will further explore the mechanism of synergetic application of porous titanium alloy with PEMF and WBV on bone repair via systematic investigation of intracellular calcium signaling in bone cells. Together, our present study aims to provide a new therapeutic strategy for bone defects in clinics, reduce the cost of the treatment of bone defects, and improve the living quality of patients with bone defects.