Kupffer cells(KC)在肝纤维化进程中发挥着重要作用，肝纤维化进展期，KC分泌TGF-β1是激活HSC增殖的始发因素，恢复期KC分泌的TRAIL、Fas是诱导HSC细胞凋亡，逆转肝纤维化的主要影响因子。大鼠腹腔巨噬细胞和肝脏KC均表达Kv1.3、Kv1.5通道，且肝纤维化不同阶段,KC中M1和M2的极化数量和Kv1.3/Kv1.5比例变化也差异明显，推测KC中Kv1.3、Kv1.5可能与肝纤维化关系密切，可能在肝纤维化不同阶段调节KC 细胞极化而影响其功能并影响HSC的增殖、凋亡。为此记录正常大鼠、肝纤维化进展期和恢复期KC的Kv1.3、Kv1.5电生理特征；通过Kv1.3、Kv1.5过表达、沉默及阻断，观察Kv1.3、Kv1.5对KC极化和分泌细胞因子及对HSC增殖、凋亡的影响，探讨Kv1.3、Kv1.5对KC细胞功能的调节机制，为肝纤维化的防治提供新的靶点。

KC play important role in hepatic fibrosis in rats. KC secretes cytokines which perform vital role in hepatic fibrosis formation and restore. KC secrete TGF-β1 is a initiated factor in the proliferation of HSC activation in hepatic progressive stage, KC secrete TRAIL、Fas and induced HSC Apoptosis in hepatic recovery stage.Kv1.3、Kv1.5 is Kv channels, involved in immunoregulation.We have found that Kv1.3、Kv1.5 exists in rat abdominal macrophage and KC,and the mRNA expression of Kv1.3、Kv1.5 are different in different stages of hepatic fibrosis.KC are polarized to subtype M1、M2 and Kv1.3/Kv1.5 ration are distinct different.we hypothesize that Kv1.3、Kv1.5 is involved in hepatic fibrosis, by regulating KC Function in different stages. We will study the the effects of Kv1.3 、Kv1.5in regulating KC secretion of cytokines and in appoptosis of HSC using Kv1.3、Kv1.5 gene over expression,gene silencing, and channel blocker.Disclosing mechanism of regulation of KC function by Kv1.3、Kv1.5 may provide new target for hepatic fibrosis.