基于功能化金属有机框架材料的光电免疫传感平台的构建及其应用研究

胃癌是我国最常见的消化道恶性肿瘤之一，严重危害人体健康，肿瘤标志物的检测对其早期筛检、诊断及预后判断至关重要。已有检测方法存在特异性差、灵敏度低等问题，建立准确、灵敏的检测方法意义重大。本研究以金属有机框架材料(MOFs)为基础，利用其多孔、吸附性强、易修饰等特点，将其分别与导电、催化、电化学发光及荧光性能优异的材料复合以制备功能性MOFs；利用电镜、X射线衍射、红外等表征技术分析不同合成方案与条件对功能性MOFs性能的影响，选择性能优异的功能性MOFs复合材料构建光电免疫传感平台，对传感平台的灵敏度、重现性、稳定性、选择性、线性范围、检出限等进行评价与优化，并对CEA、CA19-9、CA72-4以及CA125等胃癌肿瘤标志物进行检测。本研究构建的光电免疫传感平台具有灵敏度高、特异性强、应用性广等优点，也可用于其他癌症肿瘤标志物的检测，并可为大人群肿瘤筛检提供检测方法，具有广阔的应用前景。

Gastric cancer is among the most common digestive system malignant tumor in China, which seriously endangers human health, it is crucial that determination of tumor marker for its initial screening, diagnosis and prognosis. Upon poor specificity and lower sensitivity for current detection methods, development of accurate, fast and sensitive detection methods is very important. In the present study, some materials with excellent electrical conductivity, catalysis, electrochemiluminescence and fluorescence properties are combined with metal-organic framework (MOFs) based on its characteristics of porosity and easy modification to compose multifunctional MOFs (MF-MOFs). The effect of different synthetic conditions on the performance of MF-MOFs will be assessed by electron microscope, X-ray diffraction and infrared spectrometer. The MF-MOFs with excellent performance will be selected to fabricate the photoelectrochemical immunosensing platform, which will be systematically evaluated the sensitivity, reproducibility, stability, selectivity, linear range, detection limit and optimized to detect CEA、CA19-9、CA72-4 and CA125. The proposed photoelectric immunosensing platform constructed in this study has the advantages of high sensitivity, strong specificity and wide application. Also, it can be utilized for the detection of a variety of other tumor markers, and can provide a detection method for human tumor screening and will have broad application prospects.