艾纳香为贵州特色资源，但其独立自主知识产权的活性成分抗炎药理评价研究报道极少，其作用机制、作用靶向不明确，在动物炎症性疾病的防控方面研究及应用甚少。为此，本项目拟以艾纳香油及其活性成分为研究对象，通过GC-MS联用、体内外实验分析筛选抗炎物质，从临床症状及相关指标、病理变化等评价其抗LPS致雏鸡炎症的作用，以血管内皮细胞为靶向，采用siRNA、qPCR、ELISA、Western Blot等方法综合分析其对细胞分泌炎性因子作用以及细胞炎症信号通路关键靶基因的影响。通过项目实施可揭示艾纳香油抗炎物质不清晰，以血管内皮细胞中关键基因为靶向阐明艾纳香油及抗炎物质对LPS致炎雏鸡的影响机制，深度挖掘和发现艾纳香油中新的抗炎物质，为进一步开发利用艾纳香的药用特色资源打下坚实的科学依据，为艾纳香油在动物疾病方面应用的研发打基础，为贵州省特色经济发展提供坚实的科学基础。

Blumea balsanifera DC. is a characteristic resource in Guizhou. However, there are only few literatures about its active constituent of anti-inflammatory pharmacological evaluation, let alone an independent intellectual property rights. Nowadays, both its anti-inflammatory mechanism and target are not clear, the research and application about prevention and control of inflammatory diseases in animals is less. Therefore, in this project, we intend to take volatile oil and its active constituent for research objects, screen anti-inflammatory compounds through analysis of experiments by GC-MS, evaluate the effect of volatile oil and its main active constituent on inflammation in chiken induced by LPS through Changes of clinical symptoms, related indexes of animals and pathological changes, and study the influence of volatile oil of the Blumea balsamifera DC. and its active constituent on key target of inflammatory signaling pathways and cytokines secretion in chicken vascular endothelial cell by siRNA，qPCR，ELISA，Western Blot. Hoping implementation of this project will help to reveal specified anti-inflammatory compounds and its regulatory mechanism on inflammation in chiken induced by LPS and key target gene of inflammatory signaling pathways in activated chicken vascular endothelial cell, and to further exploite and find new anti-inflammatory compounds of Blumea balsamifera DC.. Which contributes to lay a solid scientific basis for the further development and utilization of medicinal resource Blumea balsamifera DC., establish the foundation for future development and application of volatile oil in animal disease areas, and afford a new way to develop characteristic economy of Guizhou.