近来研究发现，微囊藻毒素（MCs）具有生殖毒性，造成性激素水平紊乱。然而，MCs相关的生殖内分泌干扰效应和机制的研究相对匮乏。针对这一问题，本项目以斑马鱼作为模型，MC-LR作为染毒物，通过低剂量暴露实验模拟野外染毒模式，评价MC-LR对不同发育阶段斑马鱼生殖内分泌系统的干扰效应；采用液质联用技术定性、定量分析染毒斑马鱼性腺中的MC-LR，建立MC-LR生殖内分泌干扰的剂量-效应关系；釆用实时荧光定量PCR和Western blot技术，分别从下丘脑-垂体-性腺-肝（HPGL）轴、性激素合成途径、类固醇受体介导途径系统的探讨MC-LR干扰性激素合成和作用的分子机制，并从蛋白组学水平挖掘MC-LR生殖内分泌干扰作用的靶蛋白，筛选可利用的敏感的生物标志物，建立MC-LR生殖内分泌干扰作用的评价标准和模型。研究开展对于评估MCs对水环境的危害及人类生殖健康的潜在威胁具有重要的理论和实际意义。

In recent studies, it has been found that microcystins (MCs), as a ubiquitous environmental pollutant, caused reproductive toxicity and disturbed the levels of sex hormone. However, there are very limited studies on the reproductive endocrine disruption of MCs. The specific mechanism of MCs reproductive endocrine disruption has been reported rarely. In light of the above, zebrafish were as experiment animal in this project. Through the exposure experiment of low dose of MC-LR, this project aims to 1) evaluate endocrine disrupting effects of MC-LR on the reproductive system in zebrafish of different developmental stages, 2) built the dose-effect relationship between the gonadal MC-LR concentration and endpoints of reproductive endocrine disruption based on the quantitative and qualitative analysis of gonadal MC-LR using HPLC-ESI-MS, 3) discuss the molecular mechanism for reproductive endocrine disruption of MC-LR on zebrafish from the views of HPGL axis pathway, sex hormone synthesis pathway and steroid receptors-mediated pathway using fluorescence quantitative PCR and Western blotting respectively, and further screen molecular targets and available sensitive biomarkers for reproductive endocrine disruption using proteomics technology and establish evaluation standards and models for risk evaluation of MC-LR on fish reproductive endocrine disruption. The results will try to provide scientific basis for evaluation of the potential harmful effects of MCs on human reproductive health and also for the risk management and health security of ecological environment to MCs pollution.