药物药效的准确评价对于新药开发及疾病治疗具有重要意义。基于细胞支架构建的3D细胞与类器官，相比传统2D细胞，更接近人类的在体细胞特性，有望实现更准确的药效评价。本项目基于3D细胞培养技术及微纳加工技术，提出一种用于3D细胞及类器官动态监测的三维多参数集成传感芯片。该传感芯片集成三维阻抗电极阵列和三维微电极阵列，通过研究三维微纳加工技术及纳米材料与生化特异性敏感材料的修饰技术，探索芯片的表面耦合效应及敏感机理，实现3D细胞及类器官的细胞阻抗、电生理信号及多种代谢标志物的动态检测。结合多种动态特征参数及不同药物给药，研究3D细胞及类器官响应与药效的相关性，并对比2D细胞实验结果及病人临床数据，构建并验证新型的药效评价模型。本项目将为药物分析提供一种基于3D细胞及类器官动态、多参数分析的三维集成传感芯片，从多维度实现药物药效的全面、准确评价，为新药开发及个性化精准医疗提供有效的技术手段。

The accurate assessment of drug efficacy plays important roles in pharmaceutical development and disease therapy. The scaffold-based 3D cells and organoids can exhibit cell behaviors more similar to in-vivo human cells compared to conventional 2D cells, which reveals promising applications in accurate efficacy assessments. The core technique lies on how to monitor the cell behaviors of 3D cells and organoids in a dynamic manner. Based on 3D cell culture and MEMS fabrication technology, the project proposes a 3-dimensional multiparameter integrated sensor chip for dynamic monitoring of 3D cells and organoids. The sensor chip integrates a 3-dimensional impedance sensor array and a 3-dimensional microelectrode array. By studying the 3-dimensional micro/nano fabrication techniques and the modification of nanomaterials and specific biochemical materials, and exploring the coupling and sensing mechanisms on the sensor surface, the sensor chip implements dynamic monitoring of cell impedance, electrophysiological signals and multiple metabolic biomarkers for 3D cells and organoids. By analyzing these dynamic signals combining with drug administration, the correlations between the cell behaviors and drug dose can be determined, by which novel models can be constructed and validated for drug efficacy assessment with the contrast test of 2D cells and clinical data from patients. This project aims to develop a novel 3-dimensional integrated sensor chip for on-site, dynamic, multi-parameter monitoring of 3D cells and organoids in order to evaluate the drug efficacy in a multi-dimensional and accurate manner, which provides effective tools for pharmaceutical development and personalized precision medicine.