目前慢性肾脏病发病率呈逐年增长趋势,接受替代治疗者甚多,治疗费用昂贵,给社会和家庭带来沉重的负担,严重威胁着人类健康。本研究基于中医药干预可有效延缓终末期肾病发生的临床实践和肾小球硬化病机变化规律，以"毒损肾络"理论为基础结合前期工作，建立"TGF-β通路在足细胞间充质转分化(EMT)的肾小球硬化中的响应及稳心草及其黄酮干预的可能作用"工作假说，以体外培养的足细胞及阿霉素大鼠为研究对象，采用QRT-PCR、WB、细胞免疫荧光、共聚焦等技术检测足细胞EMT的证据与TGF-β通路中关键信号分子的动态表达规律，在此基础上进一步观察足细胞间充质分化与TGF-β信号通路之间的关系。并应用以稳心草及其黄酮进行干预，通过相关性分析，拟从TGF-β通路介导足细胞EMT角度揭示肾小球硬化发生机制以及"活血消痰、化浊解毒"法对其干预的科学内涵。并为该治法的临床应用和开发防治慢性肾脏病的优势中药提供理论支撑。

The incidence of Chronic kidney disease (CKD) is increasing with years in recent years as a big threaten to human health, and brings a heavy burden to the family and the society because of its costly treatment fee and the fact that many people have to receive the replacement therapy. As we know the change of the profile of glomerular sclerosis mechanism and the clinical practice that Chinese traditional medicine could delay end-stage renal disease, and according to the theory that turbid bane causes kidney disease in our previous studies. The present study aims to detect the involvement of TGF-β signaling in the glomerular sclerosis of epithelial-mesenchymal transition (EMT) and how metacentric grass flavones affect this process. Glomerular podocytes cultivated in vitro and adriamycin administrated rats were used as the experimental objects. The techniques of quantitative real-time PCR, western blotting, cyto-immunofluorescene and confocal microscopy were carried out to investigate the dynamic expression characteristics of the key signal molecular of the TGF-β signaling mediating podocytes EMT as the prototypic inducer. Metacentric grass flavones and decocta were administered and correlation analysis was used to disclose the mechanism of glomerular sclerosis occurrence and the scientific implications of the method that "invigorating the circulation blood, infusing the turbid bane and detoxification" in the perspective that TGF-β signaling mediated the EMT, and also lay a theoretical support for clinical practice and develop a better Chinese traditional medicine for prevention and treatment of CKD.