生长因子受体结合蛋白10（Grb10），又名母源表达基因1（Meg1），是由印迹基因编码的信号衔接蛋白，在生长发育调控、细胞增殖调控、凋亡、代谢和多种信号传导通路中具有重要作用。我们前期的研究结果表明Grb10与胚胎干细胞的多能性相关并且在小鼠卵母细胞中高表达。本项目计划利用我们实验室的Grb10^flox/flox小鼠和Gdf9-Cre/ZP3-Cre小鼠建立在卵母细胞中特异敲除Grb10基因的小鼠模型，通过免疫荧光、免疫印迹、免疫组化、实时定量PCR以及高通量测序等方法对卵母细胞、着床前胚胎、胚胎干细胞、囊胚的内细胞团和滋养层细胞进行检测分析，探讨母源Grb10在小鼠早期胚胎发育和胚胎干细胞多能性维持中的作用及机制。本项目利用条件性基因敲除小鼠模型开展基因功能和分子机制的研究，不仅可以增强对哺乳动物早期胚胎发育和细胞重编程机制的理解，还可以为人类生殖健康提供理论基础。

Growth factor receptor binding protein 10 (Grb10), also known as Maternally expressed gene 1 (Meg1), is a signal adaptor protein encoded by an imprinted gene and plays an important role in the regulation of growth and development, regulation of cell proliferation, apoptosis, metabolism, and multiple signaling pathways. Our previous results indicate that Grb10 is related to pluripotent of embryonic stem cell and Grb10 is expressed highly in mouse oocytes. We plan to establish mouse model with the specifically deletion of Grb10 in mouse oocytes using Grb10^flox/flox mice and Gdf9-Cre/ZP3-Cre mice in our laboratory. To investigate the function and mechanism of maternal Grb10 in early mouse embryonic development and pluripotency maintenance of embryonic stem cells, the analysis of oocytes, preimplantation embryos, embryonic stem cells, and the inner cell mass and trophoblast cells of blastocysts were performed by Immunofluorescence, Western blot, Immunochemistry, Quantitative real-time PCR, High throughput sequencing, etc. This study of gene function and molecular mechanism using conditional knockout mouse model will not only help us to understand the mechanism of early embryonic development and cell reprogramming in mammals, but also provide a theoretical basis for human reproductive health.