中药对流感防治有其独到之处，目前中药防治流感研究集中在对抗流感病毒药物的筛选，缺乏在中医理论指导下的分子机制研究。本研究采用TLR7-/-、MyD88-/-、RIG-I-/-小鼠和正常小鼠感染流感病毒株FM1，制造小鼠流感模型，并且人为制造寒、热、湿饲养环境，使用中医治疗外感表证的三种基本治法：辛温解表、辛凉解表、祛湿解表给予治疗。使用分子生物学、流式细胞仪及激光共聚焦显微镜等检测在不同饲养环境下，上述三种解表法对流感小鼠的病毒损伤恢复重要免疫通路TLR7/NF-κB及TLR7信号通路中信号蛋白的影响；细胞质的类维甲酸诱导基因-I样解旋酶（RLHs）及RLH信号通路中信号蛋白的影响。多途径、多靶点探讨不同解表法对流感小鼠的病毒损伤恢复重要免疫通路的细胞信号调控作用。其目的是探讨环境-机体-病原体三者相互作用对流感病情本身及治疗的影响机制，初步阐明几种常用解表法治疗该病的"同病异治"科学原理。

Traditional Chinese medicine (TCM) shows its originality in prevention and treatment of influenza. Currently, the study of TCM is focusing on screening of antiviral drugs, however, the molecular mechanisms under the Chinese medicine theory guidance is still not clear. To well understand the mechanism of influenza and its therapy which is associated with the interaction between environment, host and influenza virus, and briefly explain the principle of "treating same disease with different methods" by common resolving superficies method, we will conduct TLR?/?, MyD88?/? and RIG-I?/? mice and normal mice infected by FM1 virus. Furthermore, different feeding environment of cold, hot and moist will be provided artificially, and the mice will be treated by three basic treatments according to TCM guidance for superficies syndrome of exogenous disease formula for relieving superficies syndrome with pungent and warm natured drugs, pungent and cool natured drugs and desiccating formula respectively. We will use the knowledge of molecular biotechnology, flow cytometry (FCM) and confocal microscopy to analyze the injury and restoration of TLR7/NF-κB signal pathway, signal proteins involved in TLR7 pathway, RLH signal pathway and its signal proteins effect. From such multi-way and multi-target profiling, we analyze the regulation of cell signaling in immune signal pathways promoting recovery from FM1 virus infected model treated by different resolving superficies method.