本课题将应用和完善分别针对活细胞结构变化成像和功能动态成像的荧光-无标记双模态以及钙灯笼等原创研发的超分辨率显微成像技术，描绘在细胞自噬以及迁移过程中细胞器互作网络、钙信号分子以及关键蛋白的全景图。通过实时观察自噬隔离膜与内质网、内吞体/溶酶体互作等过程，迁移体内部小泡与迁移体互作融合等过程，通过建立及应用超分辨率图像大数据智能分析平台，定量描绘自噬和迁移性胞吐过程中的细胞器互作网络的动态变化。结合生化和遗传等手段，阐明自噬和迁移体分别在细胞稳态维持以及相关疾病中的重要作用。

By applying and perfecting super-resolution fluorescence-assisted diffraction computational tomography and Ca2+ lantern superresolution microscopy innovated here, this project aims to focus on mapping out the organelle interactome during cell autophagy and migrasome fusion. By simultaneously monitoring multiple organelles, critical proteins and Ca2+ signaling in live cells, we will visualize the whole process of autophagy and migration, from the formation of isolation membrane to the autophagosome/autolysosome maturation, and from the emergence of migrasomal vesicles and their trafficking and exocytosis in the end. By developing an intellegent Terabyte superresolution imaging processing platform, we will quantitatively map out the dynamic changes of organelle interactomes during these processes. In combination with the application of genetic and biochemistry techniques, we expect to reveal the critical roles of autophagy and migrasomes in the maintenance of cell homeostasis and pathogenesis of related diseases.