舌鳞癌患者一旦发生颈转移往往预后很差，长链非编码RNA(LncRNA)在肿瘤转移过程中起关键调控作用，但其调控舌鳞癌颈转移的作用和机制尚不明确。预实验我们发现LncRNA ADAMTS9-AS2，EZH2在颈淋巴转移的肿瘤中高表达，二者呈正相关关系，与患者颈转移与临床病理分期及预后相关。进一步研究证实LncRNA ADAMTS9-AS2可结合募集EZH2，降低其表达可抑制舌癌细胞的转移,反之，可促进舌癌细胞转移。由此我们提出调控舌鳞癌颈淋巴转移的新机制：LncRNA ADAMTS9-AS2可结合募集EZH2，激活一系列下游基因的表达，从而促进舌鳞癌复发及颈淋巴转移。本研究拟进一步采用RNA pull down，高通量测序，chip,动物实验等技术，探究LnRNA ADAMTS9-AS2通过EZH2激活下游靶基因进而调控舌鳞癌细胞的转移的机制，从而发现舌鳞癌的新标记物和治疗方法

Long-term outcome of TSCC(tongue squamous cell carcinoma) patients diagnosed with lymph node metastasis was always disappointing. Regulatory function of lncRNA is of vital importance, while its role in TSCC is not well konwn. Preliminary experiment showed that the expression of lncRNA ADAMTS9-AS2 and EZH2 were significantly higher in TSCC patients with lymph node metastasis and enhanced invasion and migration ability of TSCC cells. LncRNA ADAMTS9-AS2 was positively correlated wiht EZH2, both of which are related with lymph node metastasis,clinical stage and prognosis of patients.LncRNA ADAMTS9-AS2 can bind to EZH2,changing its expression level may affect metastasis of TSCC cells. We put forward hypothesis that LncRNA ADAMTS9-AS2 can bind EZH2 and activate signal transduction and promote metastasis. In vivo experiment,RNA-pull down and chip will be conduced to prove our hypothesis.The project will identify the molecular mechanism involved in lymph node metastasis , and provide experimental foundations for development targeted therapy aimed for inhibiting lymph node metastasis.