干细胞用于关节软骨修复是最具潜力和前景的领域之一，但是，长期良好的治疗效果很难获得，最终再生软骨退变，想获得长期良好的效果需要再生软骨与宿主软骨完好整合。本研究拟用天然软骨细胞外基质构建三维多孔定向支架，结合水凝胶负载Wnt5a和BMSCs，拟构建力学性能、细胞生长环境都与天然软骨相似的仿生软骨修复材料（定向脱细胞软骨基质/BMSCs-水凝胶复合体），垂直定向支架使细胞外基质定向沉积，维持再生胶原纤维的方向，Wnt5a能抑制软骨细胞肥大，维持软骨祖细胞的软骨分化，利用缓释的Wnt5a的趋化作用，使移植的BMSCs和自体干细胞向界面迁移，利用BMSCs-水凝胶作为界面连接的桥梁，通过体外、体内骨软骨缺损模型，评估软骨修复效率、再生软骨与宿主软骨界面整合情况及作用机制（Src, PLCg1, ERK12信号通路），为临床软骨修复提供理论基础和试验依据。

Stem cells based articular cartilage repair is promising, however; no method can obtain a good long-term therapeutic effect. Eventually it leads to cartilage degeneration and osteoarthritis. The long-term good repair effect needs good regeneration of cartilage and regenerated cartilage integration to host cartilage. This study intends to use the natural cartilage extracellular matrix scaffold to construct three-dimensional porous directional scaffold, combination of hydrogel gel loading Wnt5a and BMSCs to construct quasi bionic cartilage repair material, whose mechanical properties, cell growth and natural environment are similar to natural acrtilage (directional cartilage acellular cartilage matrix /BMSCs- hydrogel complex). The vertical directional structure supports the extracellular matrix orientated deposition, maintaining the direction of regenerated collagen fiber. Wnt5a can inhibit chondrocyte hypertrophy and maintain chondrogenic differentiation of progenitor cells; using the effect of chemotaxis of the release of Wnt5a, transplanted BMSCs and autologous stem cell can migrate to the interface. Meanwhile BMSCs- hydrogel was used as the interface bridge connection. Through in vitro and in vivo bone cartilage defect model, the repair efficiency cartilage repair regeneration of cartilage and host cartilage interface integration and mechanism (Src, PLCg1, ERK12, signal pathway) were evaluated, providing theoretical basis and experimental basis for cartilage repair.