干细胞用于关节软骨修复是最具潜力和前景的领域之一，但是，当前没有一种方法能提供长期良好的治疗效果，最终再生软骨退变，导致骨关节炎发生，想获得长期并且良好的修复效果需要再生软骨与宿主软骨完好的整合。国内外尚无报道利用干细胞片复合脱细胞基质方法用于软骨界面整合和再生的新思路。本研究基于前期基础，将骨软骨脱细胞基质的软骨层微孔化，使自体来源的干细胞迁移、长入和体内释放的各种因子沉积，并复合富含胞外基质、重要细胞表面蛋白的间充质干细胞片技术，利用体外、体内骨软骨缺损模型，通过干细胞荧光示踪、分子生物学、组织学、免疫组化、偏振光、Western Blotting等评估软骨修复效率、与宿主软骨界面整合情况及软骨整合的可能机制，我们拟证实骨软骨脱细胞基质与干细胞片的结合能有效的促进软骨界面整合，并揭示软骨界面整合的SDF-1/CXCR4/R-Smads/Erk1/2可能信号通路，软骨层胶原纤维方向和界面整合能维持软骨的长期修复效果，为软骨修复的研究提供理论基础和试验依据。

Stem cells for repairing articular cartilage is one of the most potential and promising field. However; there is no method that can provide long-term and good therapeutic effect. The regenerated cartilage will degenerate eventually, thus osteoarthriris is inevitable. In order to achieve enduring healing and biomechanical competence, integration between neocartilage and host cartilage is imperative. It has not been reported that using novel stem cell sheets with abundant extracellular matrix for cartilage integration and regeneration. Based on our previous research basis, we will use microporous osteochondral decellular matrix combined with ADSC sheet, rich in ECM and surface proteins, for cartilage integration and regeneration and exploring its mechanism. The microporous cartilage layer of osteochondral ecellular matrix can guide and promote the migration, growth of autologous MSCs and the deposition of autologous secreted growth factors in vivo. The cartilage regeneration and integration will be evaluated by histology, immunohistochemistry, molecular biology and polarized light. The mechanism of cartilage integration will be detected through stem cell sheet fluorescent tracer, analyzing the related genes, proteins and signal pathways in cartilage interface and comparison to the indexes of cartilage interface integration after adding CXCR4 antagonists. We will confirm that the osteochondral decellular matrix combined with stem cell sheets can promote cartilage integration effectively, and reveal the signal pathway of the cartilage integration (SDF-1/CXCR4/R-Smads/Erk1/2). The collagen fiber orientation of surface cartilage layer and cartilage integration can maintain the long-term repair effect of cartilage repair. All this will provide a theoretical and experimental basis for the study of cartilage repair.