赤霉病是危害小麦安全生产的重要病害之一。种植抗病品种能够有效经济的控制病害发生，但常规育种进展缓慢。克隆抗赤霉病关键基因，研究抗病分子遗传机理，将其应用到小麦分子育种中，对于加速育种进程具有重要的理论指导和现实意义。本实验室在前期构建了抗赤霉病小麦地方品种望水白及其Fhb1缺失的感病突变体受赤霉菌诱导的基因表达谱，筛选并克隆到一个Fhb1位点上的抗病候选基因TaRLK-B。本项目拟以该基因为研究对象，利用同源克隆、关联或连锁分析等研究TaRLK-B序列特征与Fhb1位点及抗病功能的关系；在不同抗感小麦品种中对TaRLK-B等位基因进行表达分析，研究其表达特征与功能的关系；利用瞬时沉默以及转基因等功能验证，明确该基因在抗赤霉病过程中的作用及其介导的抗病信号通路。预期研究成果可为抗赤霉病育种提供新的基因资源和分子标记，并丰富抗赤霉病分子机制理论。

Fusarium head blight (FHB) or scab is an important disease of wheat in warm and humid regions which is a serious threat to grain yield, quality and food safety. Selection of wheat cultivars resistant to FHB is the most economical and effective means for disease control. However, research on cloning of resistance related genes and characterization of molecular resistance mechanism is quite limited. In the previous research, by fast neutron irradiation, a mutant namely NAUH117 from Wangshuibai with increased FHB susceptibility, and the mutant had a 3BS chromosome fragment deletion including the Fhb1 region. Gene expression profiling for Wangshuibai and NAUH117 when induced by F. graminearum were abtained and compared in the investigation of pathways and genes associated with FHB resistance. A receptor-like kinase gene TaRLK-B from Wangshuibai was cloned as resistant candidate gene for Fhb1. In the proposed research, the function of TaRLK-B in resistance to FHB will be analyzed. We study the relationship between gene structure and the function by cloning the TaRLK-B homolog genes from species of different resistant levels. We will also study the gene expression patterns for TaRLK-B from these different wheat varieties in the survey whether this gene is specificly induced under pathogen infection in the resistant genotypes. The function of TaRLK-B in resistance to FHB will be finally analyzed by over-expression via transformation and gene silencing using VIGS,etc. The objective of the work is (1) to characterize this TaRLK-B gene in the role of resistance to FHB; (2) to provide new resistant gene resource which has the potential use in enhancement of wheat resistant to FHB and (3) to develop molecular marker for marker-assisted selection.