随着商业化转Bt基因作物大面积的推广和应用，其对农田生态系统的潜在危害日益引起人们的关注。国内外大量研究结果表明，转Bt基因作物表达的毒蛋白会以不同方式释放到土壤并在土壤中长期残留，从而对土壤生态系统造成影响。转Bt基因作物的秸秆残茬带毒量巨大，Bt络合金属离子后，所产生的络合物在土壤微生物界面的迁移行为，对后续秸秆堆肥化过程的有效进行和土壤生态安全考量具有重要影响。本研究拟考察Bt毒素与金属离子络合后在土壤微生物界面的迁移及微生物菌群变化，从而拟阐明土壤与微生物界面上生物毒素与金属离子复合作用的环境行为及关键功能微生物的响应机制。

With the popularization and application of commercial Bt transgenic crops in large areas, the potential hazards to the farmland ecosystem have attracted more and more attention. A large number of studies at home and abroad show that the toxic proteins expressed in transgenic Bt crops can be released into soil in different ways and remain in soil for a long time, thus affecting the soil ecosystem. The residues of Bt transgenic crops had a large amount of toxic residues. The transference of Bt protein complex with metal ions on soil and microbial interfaces raises a concern on the subsequent straw composting process and the safety considerations of soil ecosystem. This study intends to investigate the transference of Bt toxin complex with metal ions on soil-microorganisms interface, and then further discussing the variation of microbial flora, so as to reveal that the environmental fates of Bt toxin complex with metal ions, as well as clarify the response mechanisms of key functional microorganisms in soil system.