随着农业生物技术的快速发展，一些生物毒素在土壤中的残留可能会对土壤环境质量造成威胁。转Bt基因作物在长期种植过程中，所分泌的大量Bt毒素会与土壤活性颗粒发生紧密结合而难以被降解。因此Bt毒素在土壤颗粒表面的吸附解吸行为将是决定该毒素归趋模式的重要因素。生物表面活性剂的介入会改变蛋白毒素在颗粒界面上的吸附行为。本研究从转Bt基因秸秆中分离提取毒素蛋白，经检测其表面疏水性及基本结构性能，将其用于土壤颗粒表面的解吸行为研究，考察生物表面活性剂浓度影响土壤颗粒中Bt毒素解吸行为的特征和规律；探讨表面活性剂分子结构对Bt毒素的作用机制。在此基础上进一步深入分析颗粒孔隙液相与颗粒固相界面间Bt毒素和表面活性剂胶束缔合的规律，测定其微环境效应特征，解析生物表面活性剂影响Bt毒素解吸过程的分子机理。研究结果拟为土壤环境中Bt毒素的行为调控奠定理论基础，为生物表面活性剂在Bt毒素控制领域的应用提供科学依据。

With the rapid development of agricultural biotechnology, some kinds of biotoxins residues in soil environment will cause threat to soil quality. The transgenic Bt crops will release significant amounts of Bt toxins during long term planting, while the Bt toxins will easily combined with the soil active particles and which resulted to be hard degradation. So the adsorption and desorption process are the keys affecting the fate of Bt toxins on granule surfaces in soil. The addition of biosurfactant will change the protein toxins adsorption process at granule interfaces. After the acquisition of Bt toxins from transgenic plants, the surface hydrophobicity and basic structures properties of the toxins will be identify. Then this research will focus on the effects of biosurfactants concentration and molecular structures on Bt toxins, during the desorption process on soil particle surfaces. In order to clarify the desorption process of Bt toxins affected by biosurfactnats and to understand the molecular interaction mechanisms between them, the characteristics of Bt toxins and biosurfactant complexion will also be determined, and the micro-environmental effects investigated. The results are expected to lay the groundwork for regulation of Bt toxins behavior in soil environment and for application of biosufactants in Bt toxin control.