磷酸酶和酪氨酸激酶是生物体内一对极为重要且典型的功能相反的酶。其功能失调和癌症及其他炎症的发生密切相关。因而，找到一种分析方法实现对这一对酶的活性进行实时、定性、和定量检测显得尤为重要。目前已有报道利用其功能相反的性质设计了一些酶控组装/解组装的体系。但是反过来，该自组装体系并没有被利用于对这一对酶活进行定性和定量测定。现有的方法多集中于传统的荧光方法，并且大多需要使用复杂修饰的纳米粒子。基于这一现状，我们提出一种利用19F NMR/MRI来实时检测磷酸酶和酪氨酸激酶的新方法。设计一组含氟原子的功能化小分子水凝胶前体化合物。该类化合物可以分别对磷酸酶和酪氨酸激酶响应发生组装和解组装水凝胶，从而导致其19F NMR/MRI信号由于横向驰豫的原因而相应“关”和“开”。利用中科院强磁场科学中心的600 MHz固体核磁及其微成像系统，我们可实现对这对重要酶的活性进行进行实时的定性和定量检测。

The classic pair of phosphatase (for dephosphorylation) and tyrosine kinase (for phosphorylation) is of vital importance in regulating cellular communication, gene expression, cell growth, and cell death. Disorder of this enzyme pair would induce the occurrence of immunological diseases and cancer. Therefore, it is urgently needed to develop a new analytical method for qualitative and quantitative detection of these two enzymes in situ. Several researches have reported using the dephosphorylation and phosphorylation of these two enzymes to develop enzymatic self-assembly/disassembly systems. However, they were not employed for qualitative and quantitative detection of these two enzymes vice versa. Current methods for sensing of these two enzymes are mostly fluorescence, and nanoprobes-based. Herein, we propose a new method for qualitative and quantitative analyses of these two enzymes in situ using 19F NMR/MRI. We will develop series of 19F hydrogelators and use phosphatase/kinase to self-assembled/disassemble hydrogels. Self-assembly and disassembly of hydrogels will lead to “off” and “on” 19F NMR/MRI signals due to the transverse relaxation of the 19F nuclei spin. Using the 600 MHz NMR/μMRI system of High Magnetic Field Laboratory (HMFL) of Chinese Academy of Sciences (CAS), we will fulfill the qualitative and quantitative detection of these two important enzymes in situ.