建立针对污水厂二级出水抗性基因（ARGs）去除的再生水净化工艺，是控制再生水质风险、实现再生水安全回用的重要前提。本项目以水中典型ARGs、生物粉末活性炭（BPAC）、膜表面的界面相互作用为依据，综合利用分子生物学解析和颗粒物界面表征手段，研究ARGs在BPAC表面的吸附、微生物富集与转化以及超滤（UF）过滤去除过程，阐明ARGs胁迫条件下BPAC微生物菌群结构演变规律，明确不同形态ARGs、不同表面特征BPAC与UF的界面相互作用，揭示典型ARGs在BPAC-UF体系的水平转移机制和去除机制，建立再生水ARGs风险控制与安全回用工艺模式。本项目将为再生水深度净化与水质安全保障提供科学依据与基础性支持。

The development of reclaimed water treatment process on the basis of antibiotic resistance genes (ARGs) removal is fundamental to control water quality risks and to achieve the safe water reclamation. This project focuses on the interfacial interactions between typical ARGs, biological powdered activated carbon (BPAC) and ultra-filtration (UF) membrane, and investigates the adsorption, biological enrichment and degradation of ARGs on the surfaces of BPAC and the removal of these particulates by UF filtration. Moreover, this project uses different molecular biological methods and interfacial characterization techniques to indicate the transformation and composition of different bacteria on the surfaces of BPAC under ARGs stress, and to illustrate the interactions between ARGs, BPAC and UF. Furthermore, this project illuminates the horizontal gene transfer and removal mechanism of ARGs in the BPAC-UF process. On the basis of the above mentioned research, the BPAC-UF treatment process for ARGs risk management is proposed. This project provides scientific and fundamental support for the advanced treatment of reclaimed water and the assurance of safe water reclamation.