光感受器细胞分化效率低是限制光感受器细胞移植治疗视网膜变性的重要原因之一。近年研究发现胰岛素瘤相关蛋白1（Insulinoma associated-1, INSM-1）可以促进视网膜祖细胞（retinal progenitor cell, RPC）向光感受器细胞分化，抑制RPC向胶质细胞分化，但Insm1对视网膜祖细胞向光感受器分化的作用及其机制仍知之甚少，为此，我们提出假说：.Insm1是RPC向光感受器细胞分化的调控因子，其可能通过抑制Wnt信号通路进而促进RPC向光感受器细胞分化。为验证这一假说，我们将采用流式细胞术、实时定量聚合酶链式反应、蛋白免疫印迹法等技术手段，检测Insm1对RPC向光感受器细胞分化的调控作用，研究Insm1在促进RPC向光感受器分化的调控机制，本课题以Insm1促进RPC向光感受器细胞分化为切入点，为RPC高效定向分化为光感受器细胞的研究提供新思路。

The low efficiency of photoreceptor cell differentiation is one of the important reasons of limiting photoreceptor cell transplantation for the treatment of retinal degeneration. In recent years, it has been found that Insulinoma associated-1 (INSM-1) can promote retinal progenitor cell (RPC) differentiation into photoreceptor cells, inhibit RPC differentiation into glial cells. The role of Insm1 in the differentiation of retinal progenitor cells to photoreceptors and its mechanism is not clear, We put forward the hypothesis:.Insm1 is a regulatory factor for the differentiation of retinal progenitor cells into photoreceptor cells, which may promote the differentiation of retinal progenitor cells into photoreceptor cells by inhibiting Wnt signaling pathways. To verify this hypothesis, we will use the flow cytometry, real-time quantitative polymerase chain reaction, protein immunoblotting and other technical means to detect regulation of the retinal progenitor cells to photoreceptor cell differentiation by Insm1, and the mechanism of Insm1 promoted retina progenitor cells differentiated into photoreceptors. In this study, Insm1 was used to promote RPC differentiated into photoreceptor cells as the entry point, which provided a new idea for RPC efficient differentiation into photoreceptors. It is unclear as to why PRP not given prior to AGV implantation. please mention the reasons.