HABP1 分子靶点成像：乳腺癌及卵巢癌精准诊疗新路径

分子靶点成像在乳腺癌及卵巢癌精准诊疗中的重要作用日益彰显，因此针对特定靶点的新型诊疗探针亟待研发。透明质酸结合蛋白1（HABP1）是细胞外基质中重要的蛋白多糖，其过度表达在乳腺癌和卵巢癌的发生、发展及转移过程中起重要作用，因此是治疗及预后评价的重要因子和研究的重要靶点。目前，针对HABP1表达情况的诊断方法主要在体外。针对此现状，本课题将以HABP1为靶点，用核素99mTc标记HABP1小干扰RNA（siRNA）合成高特异性siRNA分子探针，通过一系列体内、体外研究探讨分子探针在荷瘤裸鼠模型显像的可行性，在活体肿瘤模型上评估HABP1基因表达量，着重分析该探针在肿瘤内聚集程度与HABP1表达相关性。进一步把HABP1分子靶点成像应用于肿瘤模型的放化疗敏感性研究以评价其临床价值。本课题有望为乳腺癌、卵巢癌精准诊疗提供一个新路径，有良好的临床应用前景。

The role of molecular target imaging in the accurate diagnosis and treatment of breast cancer and ovarian cancer become more and more significiant,therefor, for a specific target of new diagnostic and therapeutic probes need an urgent development. Hyaluronic acid binding protein 1 (HABP1) is an important proteoglycan in the extra-cellular matrix,over expression of HABP1 plays an crucial role in the beginning, progression and metastasis of breast and ovarian cancer. Therefore, it is not only an important element in treatment and prognosis evaluation but also a crucial target in the study.At present,the diagnosis methods of HABP1 expression are mainly in vitro.In this study, HABP1 was used as a target, and 99mTc-labeled HABP1 small interfering RNA (siRNA) was used to synthesize highly specific siRNA molecular probes.And through a series of in vivo and in vitro studies,explore the feasibility of the molecular probes in a tumor-burdened nude mice model of imaging,and the expression of HABP1 gene evaluated on a living tumor model.Emphatically,it should analyze the probe aggregation degree correlation with HABP1 expression in tumor.Furthermore,the HABP1 molecular target imaging was applied in the study of radiotherapy and chemotherapy sensitivity tumor models in order to evaluate its clinical value.This subject is expected to provide a new pathway for the accurate diagnosis and treatment of breast cancer and ovarian cancer,and have a good clinical application prospect.