研究脓毒症（Sepsis）引起的器官衰竭的分子机制的重要切入点是研究血管内皮细胞中蛋白质组及磷酸化蛋白质的修饰状态及其相互作用。然而，深入观测Sepsis血管内皮细胞磷酸化修饰水平成为研究的重难点，主要由于缺乏对病变细胞的有效筛选手段和针对细胞内磷酸化蛋白质的高效分离介质。针对以上问题，本项目利用胍基对于磷酸化肽的高富集能力、RGD序列对于Spesis病变细胞的特异性识别作用以及智能光响应主客体分子的特性，设计了一种新型双功能化磁小体复合材料，用于构建磁性固相微萃取技术和磁性细胞分选技术的平台。结合质谱检测手段，对Sepsis血管内皮细胞进行深度蛋白组学和磷酸化蛋白组学的层面分析研究，通过整合多个组学数据集来共同揭示Sepsis的进展过程中新的蛋白和分子网络，为理解Sepsis导致的多器官衰竭提供更加丰富可靠的蛋白层面实验证据。

Protein phosphorylation regulation is a very complex cascade reaction process and abnormal phosphorylation regulation can lead to many human diseases. Phosphorylated proteins play an important role in the pathways associated with sepsis. As a significant juncture, research on the modification status and interaction of proteome and phosphorylated proteins related to sepsis vascular endothelial cells was performed as a part of the study. Combination with mass spectrometry， the development of highly selective and sensitive enrichment strategies is a key problem in proteomics research. In view of the important problems in protein phosphorylation and specific cell sorting, a photo-response magnetosome material based on the host-guest interaction of cyclodextrin and azobenzene was designed as an ideal magnetic medium for magnetic solid phase microextraction as well as magnetic cell separation. Combined with MS analysis, the established method will be applied into the determination of phosphorylated proteins in sepsis vascular endothelial cells. Furthermore, to identify the possible molecular mechanism of organ failure caused by sepsis provides more abundant and reliable protein-level experimental evidence for further understanding of multiple organ failure caused by sepsis. Hence, this study will be not only conducive to the screening and early diagnosis of breast cancer, but also has important scientific significance as well as social value.