Ross"损伤反应"学说认为血管内皮炎性损伤是动脉粥样硬化（AS）形成的重要因素。前期实验显示：①在oxLDL的刺激下，蛋白激酶C与NADPH氧化酶相互作用介导的氧化应激炎症反应会促进AS的恶化；②脑络通（国医大师周仲瑛教授创立，主要成分：首乌、黄精、海藻、水蛭、三七、川芎）能够降低血管组织中CD40/CD40L mRNA的表达，但其分子机理尚未充分阐明。本研究拟采用ApoE-/-小鼠、血管内皮细胞损伤模型和HEK293模式细胞，应用RT-PCR、Western blot、免疫沉淀、体外过表达、siRNA等手段，观察脑络通如何干预蛋白激酶C与NADPH氧化酶膜蛋白复合体的激活及其传递的细胞信号，包括：①核因子-κB和p38蛋白激酶的激活，及其下游炎症因子如CD40/CD40L、MMPs的高表达；②核因子Oct-1及其调控的细胞色素P450的高表达，P450负责催化血管平衡因子EDHF的合成

The latest investigations have shown that endothelial cell inflammatory lesions are the foundation of atherosclerosis (AS). Upon the stimulation of oxLDL, the interaction between PKC and NADPH oxidase mediated inflammation mutually promote vessel wall matrix reconstruction, leading to the further deterioration of disease. Zishenyanggan Huatanxiaoyu therapy proposed by professor Zhou ZhongYing has been used as the classic therapy for AS. The previous reports have shown that Zishenyanggan Huatanxiaoyu prescript (Radix Polygoni multiflori Preparata, Sealwort, Sargassum, Leech，Pseudo-ginseng，Ligusticum wallichii) can significantly improve the symptoms of AS patients, such as dizziness, brain-ming, forgetfulness, weak waist, dark red or purple tongue, reduce the whole blood viscosity, serum CRP and CD40/CD40L mRNA expressions, restrain inflammation, and narrow plaques area in patients. However, the mechanism of action remains to be further expounded. This study is desigined to determine that Zishenyanggan Huatanxiaoyu therapy has the ability to inhibit the interaction between PKC and NADPH oxidase mediated inflammation, control fiber sticky protein expression, and protect the apoE-/-mice from endothelial injury. In this program, a vatiety of assays were performed, including semiquantitative RT-PCR、Western blot、Immunoprecipitation、gene overexpression and siRNA transfection, etc. Thus, the study is expected to provide scientific proofs for the traditional Chinese medicine therapy of AS.