盐诱导超累积植物内生菌耐受季铵盐类(QACs)污染物的调节机制与同步去除重金属和QACs机理研究

消毒剂致死是阻碍超累积植物内生菌在重金属去除领域推广应用的瓶颈问题。本项目选取超累积植物龙葵内生菌作为研究对象，采用盐诱导方式增加细胞膜厚度、调变细胞膜磷脂双分子层排列结构、细胞膜上各类转运蛋白、细胞活性蛋白、信号传导激酶等膜蛋白的活性，利用HRTEM、LSCM、HPLC-MS等现代表征手段和脂类组学方法探索调节其对季铵盐类污染物（QACs）的耐受能力机制，运用EXFS、XPS和FTIR等手段揭示表面羟基、氨基、磷酸根等活性去除位点增加机理，利用D-R模型和能量位点分布模型阐明内生菌协同去除重金属和QACs原理；以超累积植物内生菌为核心，基于流化床和物质流最大原理，构建高效协同去除组合工艺。本项目将在重金属与QACs复合污染类工业废水特别是选矿废水高效生物处理的过程机理、关键技术和工艺原理上实现创新突破，为超累积植物内生菌协同去除无机和有机污染物奠定理论基础和技术支撑。

The existence of disinfectant in the environment greatly inhibits the practical application of hyperaccumulator endophytes applied heavy metals bioremediation due to its lethal toxicity. The endophytes of Solanum nigrum L., a cadmium hyperaccumulator, will be applied in this project. The salt-induced method will be used to obtain high quaternary ammonium compounds(QACs) resistance endophytes by increasing the thickness of the cell membrane, remodeling the structure of phospholipid bilayer, regulating the activities of transporters protein, cell-active protein and signaling kinases of membrane. TEM, LSCM and HPLC-MS combined with technologies of lipidomic will be used to explore the tolerance mechanism of endophytes to QACs. The increase of activated chemical groups such as hydroxy, amino and phosphate etc. will be investigated by means of TEM, LSCM and HPLC-MS. The synergetic removal mechanism of heavy metals and QACs in endophytes under the condition of inorganic salt osmotic stress will be discussed by the D-R model and the energy locus distribution model. The process for synergetic removal of heavy metals and QACs will be conducted based on fluidized bed and material flow maximum principle. Based on the innovation and breakthrough in process, mechanism, key technique and process principle for synergetic removal of heavy metals and QACs in beneficiation wastewater by hyperaccumulator endophytes, this project will build the theoretical foundation and technical support for practical application of hyperaccumulator endophytes in wastewater treatment.