对样品进行选择性吸附、富集是提高方法检测灵敏度的有效手段之一。纳米材料由于具有尺寸小、比表面积大，易于进行表面改性等特性, 使其在生物活性物质的富集和纯化中具有非常重要的应用前景。.本项目主要利用多孔氧化铝(PAA)膜的有序模板性能、易于制备复合材料以及对磷酸根的特异性吸附等特性，拟合成基于以多孔氧化膜为模板的金属氧化物复合材料，或者是固定了金属离子的PAA膜复合材料。通过对PAA膜进行改性，减少其自身的非特异性吸附。结合PAA膜以及所引入材料的两者共同的吸附性能，可进一步提高对含磷酸根基团的生物分子（包括DNA和磷酸化肽）的特异性吸附。通过研究上述复合材料对DNA和磷酸化肽的吸附性能，探讨吸附富集机理。基于机理的研究，能对PAA膜复合材料的可控合成提供理论指导，为进一步设计合成选择性更高的PAA膜复合材料奠定理论基础，从而建立一种基于PAA膜复合材料富集含磷酸根基团的生物分子的新方法。

Selective adsorption and enrichment of samples is one of the effective means for the improvement of the detecting sensitivity. Nanomaterials had a great application in the purification and enrichment of bioactive substances due to its nanosize effect, high specific surface area, and easy of surface modification. PAA membrane is one of the highly ordered templates，it is easy to fabricate composite. And it has a strong incorporation ability to the phosphate anion. Based on the above advantages, this project want to synthesize metal oxide composite, or immobilized metal ions composite based on PAA membrane. The non-specific adsorption will be reduced by modification of PAA membrane. Combined with the adsorption properties of PAA membranes and the introduced materials, the specific adsorption of bio-molecules containing phosphate (including DNA and phosphopeptide) will be further enhanced. The adsorption mechanism was discussed by studying the adsorption properties of these composite materials on phosphopeptide and DNA. The study on the adsorption mechanism can provide guidances for the controlled synthesis of PAA membrane composite. These results are given theoretic basis for further design and synthesis of highly selective adsorption capacities of PAA membrane composite. Using these PAA membrane composites, a new method of enriching bio-molecules containing phosphate will be established.