本研究以草鱼基因组BAC文库为材料，采用三维混合池策略，进行BAC克隆的高效率、高通量测序；通过计算机算法实现，从混合池中解析各个BAC克隆的短序列数据集。基于计算机图论理论，建立单个BAC克隆短序列数据集的精确拼接方法。运用这一方法，对草鱼BAC文库中的特定克隆进行测序和拼接，填补草鱼基因组框架图中的断裂区域。本研究将提供一种化繁为简的基因组测序及组装新思路，具有广泛的应用前景。

In this research, we choose grass carp (Ctenopharyngodon idella) genomic BAC library as material,use three-dimensional mixing pool strategy for high efficiency and high-throughput sequencing of BAC clones.By using computer algorithms, we parse each BAC short sequence dataset from the mixing pool. Based on graph theory in computer science, we establish an precise assembly method for short sequence data set of single BAC clone. With this method, we do sequencing and assembling for specific clones in grass carp BAC library,so as to fill the gap areas of the grass carp genome frame diagram. This research will provide a simple new idea for genome sequencing and assembling, and have widely application prospects.