多潜能干细胞（iPSC），基因组编辑，全基因组技术的完美结合，为我们提供了在相关人类细胞中模拟疾病，遗传改造及探索发病机制的难得机遇。相关技术已在许多疾病研究中获得了成功。我们利用上述技术成功探索了脊髓侧索硬化症的早期病理改变。在此基础上本项目将利用iPSC向滋养细胞分化这一成熟且简便的分化体系，从源头上从分子及细胞水平连续动态解析子痫前期这一严重危害母胎健康疾病中滋养细胞的早期病理损害及异常信号通路并进行高通量的新药筛选。本项目的完成将为子痫前期、胎儿生长受限、复发性流产，反复着床失败等滋养细胞发育异常相关疾病提供新的研究策略及治疗思路。

Perfect combination of induced pluripotent cells (iPSCs), genetic editing and whole genome technology give us opportunity to model disease，genetic modification and explore the pathogenesis. There are many successful disease modelling using the above technology. We studied the early pathology using iPSCs from ALS patients, on basis of the experience in above study, this project plans to apply iPSCs differentiation into trophoblast cells, and dynamically explore the early pathology and the abnormal pathway of eclampsia, which greatly threaten the health of mother and infant, and finally do new drug screen using high throughput technology. This study supply a novel strategy and therapy for those disease with abnormal development of trophoblastic cells including pre-eclampsia, fetal growth restriction, recurrent spontaneous abortion and repeated implantation failure!