弧菌是水生环境中分布最多的G-细菌。N-酰基高丝氨酸内酯 ( N-AHLs )是G-菌重要信号分子，近年发现它不仅在细菌间交流调控致病性，还通过MAPK、NF-κB等机制调节宿主免疫反应，已成为医学、生物学等领域研究热点。目前，对海洋致病弧菌N-AHLs作用尚知之甚少，更未见N-AHLs对机体主要免疫调节细胞——NK细胞的调控作用报道。本课题组前期研究发现，溶藻、河流弧菌等优势致病弧菌，均可引起局部组织炎症反应和全身败血症，造成多器官损伤，而菌群感应信号分子在其致病中很可能发挥重要调控作用。本研究拟在化学合成弧菌信号分子基础上，通过流式分析技术，研究弧菌N-AHLs对宿主免疫细胞特别是NK的影响，及其对NK细胞MAPK通路调控作用，阐明相关信号机制，为深入揭示多种环境下细菌感染机制提供依据。

Vibrio are the most common G- bacterial distributed in the aquatic environment. Recent studies found that the most important signaling molecules N-acyl homoserine lactones ( N-AHLs ) not only communicate pathogenicity among G- bacteria, but also effect on eukaryotic cells by MAPK and NF-κB mechanisms, such as to regulate host immunereaction,so N-AHLs has become the research hotspots in the fields of medicine and biology. Now very few is known about N-AHL molecules of pathogenic vibrio, and the regulations of N-AHLs on the immunoloregulation cells of NK have not yet been determined. Our group early studies found that, the dominant pathogenic vibrio such as vibrio alginolyticus and vibrio fluvialis would induce local tissues inflammatory reaction and total body septicemia, and result in multi- organic lesion and nonfunction, while the quorum sensing ( QS ) signaling molecules probably developed very important regulation effection during the infection process. On the base of chemosynthesis of vibrio signaling molecules (3-oxo-C6-HSL, 3-oxo-C10-HSL), we’ll plan to study the effects of vibrio N-AHLs on host immunocytes especially NK cells,and on MAPK signal transduction mechanism in NK cells, so as to provide documentation of bacterial infection mechanism of multi-environment, and to supply a new inspiration for infection diagnosis, treatment and antimicrobial resistance control.