由小麦条锈菌引起的小麦条锈病是小麦生产上的重要病害之一，其频繁的变异常导致小麦品种抗病性的“丧失”，引发病害流行。小麦条锈菌为活体营养专性寄生真菌，已证实其有性繁殖阶段可在转主寄主小檗上完成。但其有性繁殖群体对小麦条锈病发生和流行的影响尚未见报道。云南小檗种类繁多，分布广泛，且是我国小麦条锈菌重要的越夏菌源地之一。本项目基于长期对云南小麦条锈菌越夏区域的调查和检测，拟利用高信息量的ISSR分子标记，能有效分析小麦条锈菌基因型的SSR标记、贝克曼库尔特CEQ8800 DNA分析系统分析有小檗的越夏地区（有性繁殖）、无小檗的越夏区（无性繁殖）和非越夏区（小麦条锈病主发区）三个不同区域内和区域间小麦条锈菌的群体结构关系，探寻有性繁殖对小麦条锈菌变异的影响；并拟通过比较跨年度间各区域小麦条锈菌的群体结构差异，分析小麦条锈菌的有性繁殖对小麦条锈病发生的影响，为从源头治理小麦条锈病提供参考。

Wheat stripe rust disease, caused by Puccinia striiformis f. sp. tritici (PST), is an economically important disease of wheat-growing regions in China. The rapid pathogen variability results in "resistance loss" of wheat cultivars, and leads to the frequent epidemic of the disease. PST is an obligate parasitism fungi. It has been identified that sexual reproduction of PST is on the barberry (Berberis spp.), and the barberry is as alternate host of the stripe rust fungi. It has not reported that sexual reproduction population of PST effected on the occurrence and epidemic of wheat stripe rust. Barberry was more kinds and widely distributed in Yunnan. And Yunnan is one of the most important over-summering areas. In this project, based on accurate detection of Beckmann Coulter CEQ8800 DNA Analyzer and large amount of information of ISSR and SSR molecular marker, Population structure will be analyzed between over-summering (having the Berberis zones and non-Berberis zones) and prevent area. It will be explored sexual production effects on variability of PST. Sexual production affection on the happen of wheat stripe rust through the spatial population structure analyzed within locations across locations and over years. Above all, it will offer scientific evidence to control wheat stripe rust disease.