饲粮不同能量来源对断奶仔猪肠道功能和粘膜更新的影响及机制研究

肠道粘膜上皮的快速增殖为仔猪断奶应激等肠道损伤修复提供了基础，同时也需要大量养分氧化代谢提供能量。氨基酸、葡萄糖和脂肪酸作为肠道上皮的主要供能物质，它们在断奶仔猪肠道的利用和代谢研究对仔猪断奶期的营养调控具有重要意义。本研究组前期对仔猪肠道上皮更新过程的蛋白质组学结果表明仔猪断奶后影响了绒毛细胞和隐窝细胞能量代谢，但不同的能量来源对断奶仔猪肠上皮细胞更新的影响还不清楚。因此本项目拟对断奶仔猪添加不同能量来源（谷氨酰胺、葡萄糖和脂肪酸），研究其对断奶仔猪肠道形态和功能、肠上皮细胞更新的影响，并结合蛋白质组学和代谢组技术，探讨了不同能量来源对断奶仔猪肠道上皮更新影响的分子机制。为阐明断奶仔猪肠道养分代谢及营养需要，调控仔猪断奶期肠道营养、缓解断奶应激提供理论依据。

The rapid proliferation of intestinal epithelial provides a basis for intestinal damage repair (e.g. weaning stress), and requires energy by oxidative metabolism of nutrients. Amino acids, glucose and fatty acids as the main energy supply substances of enterocytes, their metabolism on intestinal epithelial cell has important implications for the nutritional regulation of weaning piglets. Our group has studied on intestinal epithelial renewal process by proteomics and the results show that weaning changed the energy metabolism on villus and crypt cells of piglets, but the effect of different energy sources on intestinal epithelial cell renewal is still unclear in weaning piglets. Therefore, this project intends to investigate the different energy sources (glutamine, glucose and fatty acids) on the morphology and function of intestine and epithelial cell renewal on weaning piglets. And the results will disclosure the mechanisms of different energy sources on mucosal turnover of weaning piglets through proteomics and metabonomics. Our results will be helpful to provide the intestinal nutrient metabolism and nutritional needs on weaning piglets, and theoretical basis for weaning stress.