肌肉因子白介素-15在运动减轻肥胖中的作用及机制

肥胖是由特定的生化因子引起的一系列进食调控和能量代谢紊乱的疾病，适当的运动是肥胖防治最积极有效的方法。在前期工作观察到运动促进肥胖大鼠骨骼肌白介素-15（interleukin,IL-15）表达和合成的基础上，本项目拟于在体和离体鼠的肥胖模型上，观察运动对骨骼肌内源性IL-15的表达、合成及分泌的影响；于在体和离体IL-15敲除鼠的模型上，以体脂百分数、脂肪细胞的分化、脂质的沉积和分解为指标，观察运动减轻肥胖的作用是否由IL-15所介导；以原代培养的骨骼肌细胞IL-15的表达和活性为指标，观察运动调节IL-15表达的作用机制，以论证运动通过促进骨骼肌内源性IL-15的分泌减轻肥胖的假说，探讨运动作用的分子机制，为运动减轻肥胖提供直接的理论依据。

Obesity has been considered as a real disease which is a complex disorder of appetite regulation and energy metabolism. Appropriate exercise is the most positive and effective methods of obesity prevention. In preliminary work, we found treadmill running promoted the interleukin-15 (IL-15) expression and synthesis in skeletal muscle in high fat induced obese rats. In present project, we observe the change of skeletal muscle endogenous IL-15 expression, synthesis and secretion during exercise in vivo and in vitro obese model, investigate the alteration of body fat percentage, adipose cell differentiation and lipid deposition and decomposition during exercise in vivo and in vitro IL-15 knockout model and test whether the effect of exercise on weight loss is mediated by IL-15. In addition, we aim the change of IL-15 expression and activity in primary cultured skeletal muscle cells and elucidate the mechanisms of exercise. On the basis of the above study, we want to demonstrate that the weight loss induced by exercise is mediated by promotion IL-15 secretion in skeletal muscle, explore the molecular mechanisms of exercise, and provide direct theoretical basis for the effect of exercise on weight loss.