口蹄疫严重危害世界各国畜牧业发展，造成巨大的经济损失。目前我国采取以强制免疫注射为主的综合性防控措施来预防和控制口蹄疫，急需研发更加安全、高效的疫苗及免疫佐剂，有效防控口蹄疫疫情发生。本项目以口蹄疫各病毒蛋白中辅助性T细胞表位(Th表位)为研究对象，结合生物信息学分析方法，采用化学合成技术合成口蹄疫病毒候选Th表位多肽。通过建立猪SLA-Ⅱ类限制性表位体外鉴定平台及Th表位肽活性测定方法鉴定FMDV Th细胞表位，绘制FMDV Th表位图谱。同时，将鉴定的Th表位应用组合型设计理论和方法制备成新型T细胞免疫佐剂，配制疫苗免疫猪群，测定其体液免疫应答和细胞免疫应答水平，评价新型T细胞免疫佐剂的免疫效果。该研究有望增强免疫动物的体液免疫应答并弥补细胞免疫应答不足的缺陷，为研制能同时激发特异性体液免疫和细胞免疫应答的口蹄疫新型疫苗及免疫佐剂奠定研究基础，具有重大的社会经济意义和应用前景。

Foot-and-mouth disease (FMD) causes serious harm to the development of global animal husbandry, resulting in huge economic losses. At present, we adopt a mandatory immunization-based comprehensive prevention and control measures to prevent and control FMD in China, which has urgent need to develop new type vaccines and immune adjuvants with more secure and high performance to prevent and control FMD outbreak effectively.. Regarding helper T cell epitopes (Th epitope) of foot and mouth disease virus proteins as the research objects in the study, we synthesized candidate Th epitope peptides using chemical synthesis combined with bioinformatics analysis methods. Th cell epitopes of FMDV were identified through the establishment of identification platform of SLA-II class (swine MHC II) restricted epitopes in vitro and activity determination method of Th epitope peptide, and then Th cell epitope maps of FMDV were draw. Moreover, new type T-cell immunoadjuvant were prepared by using combinatorial design theory and methods based on identified Th epitope, and swine were immunized with vaccine contained T-cell immunoadjuvant. The humoral immune response and cellular immune response level of immunized swine were determined and immune effects of the new T-cell immune adjuvants were valuated. The study is expected to enhance the humoral immune responses and make up the shortcoming of lack of cellular immune responses of immune animals, and lays the research foundation for the development of new type FMD vaccine and immune adjuvant that stimulated humoral immune response and cellular immune responses simultaneous, which has significant social and economic significance and application prospects.