菌根是土壤真菌与植物形成的共生体，大约有80%的陆生植物能够形成菌根。丛枝菌根是最古老的 （距今已有4亿年）的真菌-植物共生体。菌根的形成是一个非常复杂的微生物植物相互作用过程，关于其共生关系建立的分子机制的研究已经成为该研究领域最活跃的热点问题。本项目拟在研究共生体形成早期共生双方在分子水平上的相互作用以及摩西球囊霉和荷兰芹共生早期的抑制差减杂交文库中Gm201基因的基础上，利用realtime-PCR研究基因的表达调控；利用酵母双杂研究蛋白质体内外相互作用以及利用宿主诱导的基因沉默和酵母突变株的功能互补进行基因功能验证。研究该基因在共生早期信号转导方面的调控机制，为AM真菌与宿主植物早期共生关系的建立提供新的分子生物学证据，进一步阐明AM真菌与植物共生体形成时相互识别的信号传导过程。

The arbuscular mycorrhizal(AM)symbiosis is the association between fungi and the roots of terrestrial plants . AM fungi are associated with 80% of terrestrial plants.Yet，molecular mechanisms leading to signaling events and appressorium formation are just beginning to be unravelled. In this study, gene Gm201 was selected from SSH library in vitro system of Glomus mosseae and Petroselinum crispum. The full length cDNA sequences of Gm201 was obtained. In view of the above, we are going to carry out the research on investigating the role of gene Gm201 in the molecular responses during early signal transduction .The expression of Gm201 is analyzed by real-time PCR; proteins interaction was studied by yeast two-hybrid; host induced gene silence and mutant yeast strains was used to study the functions of Gm201.This study will privide a better understanding of the signaling mechanisms involved in the endosymbioes,and also open the way to study the molecular mechanasims leading to its formation.