嵌入式Nafion-多壁碳纳米管粉末微电极的磺胺类兽药残留暂态电化学检测机理研究

由于滥用和非法使用违禁药物，造成动物源性食品磺胺类兽药残留超标问题比较突出。标准的磺胺类药物残留检测如液相色谱-串联质谱法虽然方法灵敏、准确，但仪器贵重、检测成本高、操作繁琐等不适于现场检测，因此，发展低成本、方便携带、可靠的现场检测方法是监控兽药残留的重要手段之一。本研究利用铂（Pt）微电极作为基底电极，简化三电极为两电极系统，针对其电催化活性低和选择性差的不足，利用多壁碳纳米管（MWCNTs）高比表面积、高电导率特性和Nafion对阳离子的选择性，制备嵌入式Nafion-MWCNTs粉末微电极（PME），既能克服常规电极受到稳态的制约，又可实现在线清洗，避免长期使用带来的稳定性低、重复性差等。利用制备的嵌入式Nafion-MWCNTsPME，基于暂态电化学响应机理，结合自行设计的便携式电化学检测装置，建立一种适用于现场快速检测磺胺类兽药残留的方法。

Due to abuse and illegal use of drugs, the super standard of sulfonamides residues in animal derived food was more outstanding. The standard detection method for sulfonamides residues such as liquid chromatography-tandem mass spectrometry was sensitive and accurate, but this technique required large valuable instrument and the detecting procedures were high cost and complicated etc., was not suitable for the field detection. Therefore, the development of field detection method as well as advantages of low cost, easy to carry and reliable was very important to monitor food veterinary drug residues. In this study, platinum (Pt) microelectrode was used as basal electrode, the three electrodes systems could be simplified as two electrodes systems. In view of its low electricity catalytic activity and poor selectivity, based on the high specific surface area, high conductivity of multi-wall carbon nanotubes (MWCNTs) and Nafion cationic selectivity, the embedded Nafion-MWCNTs powder microelectrode (PME) was fabricated. This PME could overcome the restriction of the steady-state for inconventional electrodes, and could realize online cleaning, avoid long-term use of low repetitive and poor stability etc. According to transient electrochemical principles，using the embedded Nafion-MWCNTsPME, which combined with the home-made portable electrochemical detection device, a new method for field detecting sulfonamides residues was established.